Hemorrhagic shock and surgical stress alter distribution of labile zinc within high-and low-molecular-weight plasma fractions

Edward Kelly, Jeff Mathew, Jonathan E. Kohler, Amy L. Blass, And David I. Soybel

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Zinc ions (Zn2+) are essential for tissue repair following injury or stress. We hypothesize that during such stresses Zn2+ is redistributed to labile pools in plasma components. Here we tested this hypothesis using a novel assay to monitor labile Zn2+ in plasma in hemorrhagic shock. Adult rats in the shock group (S group) underwent hemorrhage and resuscitation. Blood samples were drawn at baseline and at 1, 4, and 24 h. The surgical control group (SC group) was anesthetized and instrumented, but not bled. Albumin, total Zn2+, and labile Zn2+ levels were assayed in plasma. Binding capacity for Zn2+ was assessed in high-and low-molecular-weight pools. Significant decreases in total Zn2+ were observed by 24 h, in both S and SC groups. Albumin levels were significantly reduced in the S group at 1 and 4 h but restored at 24 h; significant changes were not observed in other groups. In whole plasma, labile Zn2+ levels were stable initially in the S and SC groups, but declined at 24 h. In the high-molecular-weight pool, marked and significant impairment of binding was noted throughout all time periods following the shock period in the S group. Such changes were observed in the SC group of less intensity and duration. These experiments suggest that shock alters affinity of plasma proteins for Zn 2+, promoting delivery to peripheral tissues during periods of increased Zn2+ utilization.

Original languageEnglish (US)
Pages (from-to)314-319
Number of pages6
JournalShock
Volume38
Issue number3
DOIs
StatePublished - Aug 1 2012

All Science Journal Classification (ASJC) codes

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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