Hepadnavirus genome replication and persistence

Jianming Hu, Christoph Seeger

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Hallmarks of the hepadnavirus replication cycle are the formation of covalently closed circular DNA (cccDNA) and the reverse transcription of a pregenomic RNA (pgRNA) in core particles leading to synthesis of the relaxed circular DNA (rcDNA) genome. cccDNA, the template for viral RNA transcription, is the basis for the persistence of these viruses in infected hepatocytes. In this review, we summarize the current state of knowledge on the mechanisms of hepadnavirus reverse transcription and the biochemical and structural properties of the viral reverse transcriptase (RT). We highlight important gaps in knowledge regarding cccDNA biosynthesis and stability. In addition, we discuss the impact of current antiviral therapies on viral persistence, particularly on cccDNA.

Original languageEnglish (US)
JournalCold Spring Harbor Perspectives in Medicine
Volume5
Issue number7
DOIs
StatePublished - Jul 1 2015

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Hepadnaviridae
Circular DNA
Genes
Genome
Transcription
Reverse Transcription
RNA-Directed DNA Polymerase
Biosynthesis
Viral RNA
Viruses
Antiviral Agents
Structural properties
Hepatocytes
RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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Hepadnavirus genome replication and persistence. / Hu, Jianming; Seeger, Christoph.

In: Cold Spring Harbor Perspectives in Medicine, Vol. 5, No. 7, 01.07.2015.

Research output: Contribution to journalArticle

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