Hepatic progesterone receptors: Characterization in the turtle chrysemys picta

Deborah Riley, Joseph C. Reese, Ian P. Callard

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

High and low affinity progesterone (P)-binding proteins similar to those described in the chick oviduct were characterized for the first time in liver cytosol in an oviparous turtle, Chrysemys picta. Two forms of high affinity P receptor were separated by diethyl aminoethyl (DEAE)-Sepharose anion exchange chromatography; the A form eluted in low salt buffer, and the B form at a KC1 concentration of 0.20 M in a linear gradient. After photoaffinity labeling of crude cytosol with [3H] R5020, two overlapping peaks of radioactivity were detected with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After separation with DEAE-Sepharose and photoaffinity labeling, the A form migrated as a single peak with a mol wt of 90,000, and the B form as a single peak with a mol wt of 123,000. The affinity (Kd, 2.9 × 10-9 M), capacity (1 pmol/mg protein), and binding specificity were characteristic of a P receptor. P competed most effectively for [3H]P binding. R5020 and deoxycorticosterone were good competitors; 5 -αpregnenolone, 17αhydroxyprogesterone, and testosterone were quite effective; cortisol, corticosterone, aldosterone, and 5 -αdihydrotestosterone were poor competitors. A lower affinity P-binding component (Kd, 1 × 10-7 M; maximum binding, 15 pmol/mg protein) coeluted from DEAE-Sepharose columns with the A and B receptor forms. This is the first description of a hepatic P receptor in any vertebrate.

Original languageEnglish (US)
Pages (from-to)1195-1201
Number of pages7
JournalEndocrinology
Volume123
Issue number2
DOIs
StatePublished - Aug 1 1988

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Turtles
Progesterone Receptors
Promegestone
Sepharose
Cytosol
Liver
Progesterone-Binding Globulin
Oviparity
Pregnenolone
Desoxycorticosterone
Oviducts
Dihydrotestosterone
Corticosterone
Aldosterone
Protein Binding
Sodium Dodecyl Sulfate
Radioactivity
Anions
Hydrocortisone
Vertebrates

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Riley, Deborah ; Reese, Joseph C. ; Callard, Ian P. / Hepatic progesterone receptors : Characterization in the turtle chrysemys picta. In: Endocrinology. 1988 ; Vol. 123, No. 2. pp. 1195-1201.
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Hepatic progesterone receptors : Characterization in the turtle chrysemys picta. / Riley, Deborah; Reese, Joseph C.; Callard, Ian P.

In: Endocrinology, Vol. 123, No. 2, 01.08.1988, p. 1195-1201.

Research output: Contribution to journalArticle

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