Hepatitis C viral clearance and antibody reactivity patterns in persons with haemophilia and other congenital bleeding disorders

K. Messick, J. C. Sanders, J. J. Goedert, M. E. Eyster

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    We studied hepatitis C virus (HCV) clearance and antibody reactivity patterns in a cohort of 100 haemophiliacs exposed to unsterilized blood products, of whom 25 were antiHCV negative and 75 were antiHCV positive [49 human immunodeficiency virus (HIV) negative and 26 HIV positive]. HCV RNA was measured by the 2.0 bDNA assay and an 'in-house' polymerase chain reaction assay. Antibody reactivity patterns were examined using a recombinant immunoblot assay (RIBA). Prior HCV infection was found in two (8%) of 25 antiHCV negative patients. HCV viraemia persisted in all 26 antiHCV+ patients who were coinfected with HIV. HCV RNA clearance was found in 12 (25%) of 49 antiHCV+, HIV- patients. Viral clearance was associated with younger current age (P < 0.01) and age at infection (P < 0.001), but not with duration of infection or with dose or frequency of clotting factor use. RIBA ratios reflecting an index of each patient's overall reactivity to four HCV epitopes were significantly lower in those with viral clearance (P < 0.0001). Over a period of 15 years, those with viral clearance demonstrated significant loss of reactivity to the NS3, NS4 and NS5 epitopes, while those with viral persistence demonstrated relatively stable reactivities to all epitopes. We conclude that spontaneous HCV RNA clearance in haemophiliacs is age-related and is unlikely to occur in those coinfected with HIV. The loss of antibody reactivity for some epitopes, especially c22 (core), may be a marker for the natural resolution of chronic HCV infection.

    Original languageEnglish (US)
    Pages (from-to)568-574
    Number of pages7
    JournalHaemophilia
    Volume7
    Issue number6
    DOIs
    StatePublished - Jan 1 2001

    Fingerprint

    Viral Antibodies
    Congenital, Hereditary, and Neonatal Diseases and Abnormalities
    Hemophilia A
    Hepatitis C
    Hepacivirus
    Hemorrhage
    HIV
    Epitopes
    Virus Diseases
    RNA
    Branched DNA Signal Amplification Assay
    Hepatitis C Antibodies
    Blood Coagulation Factors
    Antibodies
    Viremia
    Chronic Hepatitis C
    Infection
    Polymerase Chain Reaction

    All Science Journal Classification (ASJC) codes

    • Hematology
    • Genetics(clinical)

    Cite this

    @article{e2651dc73f6a45e38fe70799b2d4d579,
    title = "Hepatitis C viral clearance and antibody reactivity patterns in persons with haemophilia and other congenital bleeding disorders",
    abstract = "We studied hepatitis C virus (HCV) clearance and antibody reactivity patterns in a cohort of 100 haemophiliacs exposed to unsterilized blood products, of whom 25 were antiHCV negative and 75 were antiHCV positive [49 human immunodeficiency virus (HIV) negative and 26 HIV positive]. HCV RNA was measured by the 2.0 bDNA assay and an 'in-house' polymerase chain reaction assay. Antibody reactivity patterns were examined using a recombinant immunoblot assay (RIBA). Prior HCV infection was found in two (8{\%}) of 25 antiHCV negative patients. HCV viraemia persisted in all 26 antiHCV+ patients who were coinfected with HIV. HCV RNA clearance was found in 12 (25{\%}) of 49 antiHCV+, HIV- patients. Viral clearance was associated with younger current age (P < 0.01) and age at infection (P < 0.001), but not with duration of infection or with dose or frequency of clotting factor use. RIBA ratios reflecting an index of each patient's overall reactivity to four HCV epitopes were significantly lower in those with viral clearance (P < 0.0001). Over a period of 15 years, those with viral clearance demonstrated significant loss of reactivity to the NS3, NS4 and NS5 epitopes, while those with viral persistence demonstrated relatively stable reactivities to all epitopes. We conclude that spontaneous HCV RNA clearance in haemophiliacs is age-related and is unlikely to occur in those coinfected with HIV. The loss of antibody reactivity for some epitopes, especially c22 (core), may be a marker for the natural resolution of chronic HCV infection.",
    author = "K. Messick and Sanders, {J. C.} and Goedert, {J. J.} and Eyster, {M. E.}",
    year = "2001",
    month = "1",
    day = "1",
    doi = "10.1046/j.1365-2516.2001.00559.x",
    language = "English (US)",
    volume = "7",
    pages = "568--574",
    journal = "Haemophilia",
    issn = "1351-8216",
    publisher = "Wiley-Blackwell",
    number = "6",

    }

    Hepatitis C viral clearance and antibody reactivity patterns in persons with haemophilia and other congenital bleeding disorders. / Messick, K.; Sanders, J. C.; Goedert, J. J.; Eyster, M. E.

    In: Haemophilia, Vol. 7, No. 6, 01.01.2001, p. 568-574.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Hepatitis C viral clearance and antibody reactivity patterns in persons with haemophilia and other congenital bleeding disorders

    AU - Messick, K.

    AU - Sanders, J. C.

    AU - Goedert, J. J.

    AU - Eyster, M. E.

    PY - 2001/1/1

    Y1 - 2001/1/1

    N2 - We studied hepatitis C virus (HCV) clearance and antibody reactivity patterns in a cohort of 100 haemophiliacs exposed to unsterilized blood products, of whom 25 were antiHCV negative and 75 were antiHCV positive [49 human immunodeficiency virus (HIV) negative and 26 HIV positive]. HCV RNA was measured by the 2.0 bDNA assay and an 'in-house' polymerase chain reaction assay. Antibody reactivity patterns were examined using a recombinant immunoblot assay (RIBA). Prior HCV infection was found in two (8%) of 25 antiHCV negative patients. HCV viraemia persisted in all 26 antiHCV+ patients who were coinfected with HIV. HCV RNA clearance was found in 12 (25%) of 49 antiHCV+, HIV- patients. Viral clearance was associated with younger current age (P < 0.01) and age at infection (P < 0.001), but not with duration of infection or with dose or frequency of clotting factor use. RIBA ratios reflecting an index of each patient's overall reactivity to four HCV epitopes were significantly lower in those with viral clearance (P < 0.0001). Over a period of 15 years, those with viral clearance demonstrated significant loss of reactivity to the NS3, NS4 and NS5 epitopes, while those with viral persistence demonstrated relatively stable reactivities to all epitopes. We conclude that spontaneous HCV RNA clearance in haemophiliacs is age-related and is unlikely to occur in those coinfected with HIV. The loss of antibody reactivity for some epitopes, especially c22 (core), may be a marker for the natural resolution of chronic HCV infection.

    AB - We studied hepatitis C virus (HCV) clearance and antibody reactivity patterns in a cohort of 100 haemophiliacs exposed to unsterilized blood products, of whom 25 were antiHCV negative and 75 were antiHCV positive [49 human immunodeficiency virus (HIV) negative and 26 HIV positive]. HCV RNA was measured by the 2.0 bDNA assay and an 'in-house' polymerase chain reaction assay. Antibody reactivity patterns were examined using a recombinant immunoblot assay (RIBA). Prior HCV infection was found in two (8%) of 25 antiHCV negative patients. HCV viraemia persisted in all 26 antiHCV+ patients who were coinfected with HIV. HCV RNA clearance was found in 12 (25%) of 49 antiHCV+, HIV- patients. Viral clearance was associated with younger current age (P < 0.01) and age at infection (P < 0.001), but not with duration of infection or with dose or frequency of clotting factor use. RIBA ratios reflecting an index of each patient's overall reactivity to four HCV epitopes were significantly lower in those with viral clearance (P < 0.0001). Over a period of 15 years, those with viral clearance demonstrated significant loss of reactivity to the NS3, NS4 and NS5 epitopes, while those with viral persistence demonstrated relatively stable reactivities to all epitopes. We conclude that spontaneous HCV RNA clearance in haemophiliacs is age-related and is unlikely to occur in those coinfected with HIV. The loss of antibody reactivity for some epitopes, especially c22 (core), may be a marker for the natural resolution of chronic HCV infection.

    UR - http://www.scopus.com/inward/record.url?scp=0035543818&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0035543818&partnerID=8YFLogxK

    U2 - 10.1046/j.1365-2516.2001.00559.x

    DO - 10.1046/j.1365-2516.2001.00559.x

    M3 - Article

    C2 - 11851755

    AN - SCOPUS:0035543818

    VL - 7

    SP - 568

    EP - 574

    JO - Haemophilia

    JF - Haemophilia

    SN - 1351-8216

    IS - 6

    ER -