3 Citations (Scopus)

Abstract

Prelabeled rat liver nuclei were purified, detergent-rinsed to remove cytoplasmic RNA contaminants and incubated in two in vitro RNA transport assays. Appropriate compartmentation of nuclear RNA sequences was maintained in an assay containing polyvinylpyrrolidone (PVP), which prevents nuclear swelling in aqueous media. Under these conditions, poly (A)+ RNA released in the presence of PVP (and ATP) was significantly more active in directing in vitro protein synthesis than poly(A)+ RNA released in the absence of either additive. Further, the excised fifth intron of α1-acid glycoprotein remained nucleus-restricted, while release of poly(A)+ processing catabolites occurred in the absence of PVP. The appropriate compartmentation of nuclear sequences was lost following treament of rats with the hepato-carcinogen thioacetamide, paralleling the in vivo situation. This assay should prove useful for subsequent studies on nucleocytoplasmic RNA transport in vitro, in particular for investigations relating to the altered RNA transport associated with the initiation of carcinogenesis.

Original languageEnglish (US)
Pages (from-to)1235-1238
Number of pages4
JournalCarcinogenesis
Volume8
Issue number9
DOIs
StatePublished - Sep 1 1987

Fingerprint

RNA Transport
Nuclear RNA
Povidone
Thioacetamide
Messenger RNA
Poly A
Cell Nucleus Active Transport
Carcinogens
Detergents
Introns
Glycoproteins
Carcinogenesis
Adenosine Triphosphate
RNA
Acids
Liver
In Vitro Techniques
Proteins

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Clawson, Gary A. ; Button, Jane D. ; Woo, C. H. / Hepatocarcinogen-induced alterations in nuclear RNA compartmentation. In: Carcinogenesis. 1987 ; Vol. 8, No. 9. pp. 1235-1238.
@article{a5102cbb7d504cff97dfcbc4204ed252,
title = "Hepatocarcinogen-induced alterations in nuclear RNA compartmentation",
abstract = "Prelabeled rat liver nuclei were purified, detergent-rinsed to remove cytoplasmic RNA contaminants and incubated in two in vitro RNA transport assays. Appropriate compartmentation of nuclear RNA sequences was maintained in an assay containing polyvinylpyrrolidone (PVP), which prevents nuclear swelling in aqueous media. Under these conditions, poly (A)+ RNA released in the presence of PVP (and ATP) was significantly more active in directing in vitro protein synthesis than poly(A)+ RNA released in the absence of either additive. Further, the excised fifth intron of α1-acid glycoprotein remained nucleus-restricted, while release of poly(A)+ processing catabolites occurred in the absence of PVP. The appropriate compartmentation of nuclear sequences was lost following treament of rats with the hepato-carcinogen thioacetamide, paralleling the in vivo situation. This assay should prove useful for subsequent studies on nucleocytoplasmic RNA transport in vitro, in particular for investigations relating to the altered RNA transport associated with the initiation of carcinogenesis.",
author = "Clawson, {Gary A.} and Button, {Jane D.} and Woo, {C. H.}",
year = "1987",
month = "9",
day = "1",
doi = "10.1093/carcin/8.9.1235",
language = "English (US)",
volume = "8",
pages = "1235--1238",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "9",

}

Hepatocarcinogen-induced alterations in nuclear RNA compartmentation. / Clawson, Gary A.; Button, Jane D.; Woo, C. H.

In: Carcinogenesis, Vol. 8, No. 9, 01.09.1987, p. 1235-1238.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hepatocarcinogen-induced alterations in nuclear RNA compartmentation

AU - Clawson, Gary A.

AU - Button, Jane D.

AU - Woo, C. H.

PY - 1987/9/1

Y1 - 1987/9/1

N2 - Prelabeled rat liver nuclei were purified, detergent-rinsed to remove cytoplasmic RNA contaminants and incubated in two in vitro RNA transport assays. Appropriate compartmentation of nuclear RNA sequences was maintained in an assay containing polyvinylpyrrolidone (PVP), which prevents nuclear swelling in aqueous media. Under these conditions, poly (A)+ RNA released in the presence of PVP (and ATP) was significantly more active in directing in vitro protein synthesis than poly(A)+ RNA released in the absence of either additive. Further, the excised fifth intron of α1-acid glycoprotein remained nucleus-restricted, while release of poly(A)+ processing catabolites occurred in the absence of PVP. The appropriate compartmentation of nuclear sequences was lost following treament of rats with the hepato-carcinogen thioacetamide, paralleling the in vivo situation. This assay should prove useful for subsequent studies on nucleocytoplasmic RNA transport in vitro, in particular for investigations relating to the altered RNA transport associated with the initiation of carcinogenesis.

AB - Prelabeled rat liver nuclei were purified, detergent-rinsed to remove cytoplasmic RNA contaminants and incubated in two in vitro RNA transport assays. Appropriate compartmentation of nuclear RNA sequences was maintained in an assay containing polyvinylpyrrolidone (PVP), which prevents nuclear swelling in aqueous media. Under these conditions, poly (A)+ RNA released in the presence of PVP (and ATP) was significantly more active in directing in vitro protein synthesis than poly(A)+ RNA released in the absence of either additive. Further, the excised fifth intron of α1-acid glycoprotein remained nucleus-restricted, while release of poly(A)+ processing catabolites occurred in the absence of PVP. The appropriate compartmentation of nuclear sequences was lost following treament of rats with the hepato-carcinogen thioacetamide, paralleling the in vivo situation. This assay should prove useful for subsequent studies on nucleocytoplasmic RNA transport in vitro, in particular for investigations relating to the altered RNA transport associated with the initiation of carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0023213363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023213363&partnerID=8YFLogxK

U2 - 10.1093/carcin/8.9.1235

DO - 10.1093/carcin/8.9.1235

M3 - Article

C2 - 2441886

AN - SCOPUS:0023213363

VL - 8

SP - 1235

EP - 1238

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 9

ER -