Herpes simplex latent infection

Quantitation of latency-associated transcript-positive neurons and reactivable neurons

Richard Tenser, W. A. Edris, K. A. Hay

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dorsal root ganglion neurons, which express herpes simplex virus (HSV) latency-associated transcript (LAT) during experimental latent infection, were investigated by in situ hybridization. The number of LAT-positive neurons was determined by examination of ganglion serial sections. In other latently infected mice, the number of ganglion neurons that reactivated HSV antigen after explant culture was determined in serial sections. LAG was detected in 100 percent of ganglia, with an average of 19.5 LAT-positive neurons per ganglion. After explant culture of latently infected ganglia (in the presence of colchicine to decrease spread of reactivated virus), HSV antigen was detected in 94 percent of ganglia, with an average of 13.1 positive neurons in the antigen-positive ganglia. The similar quantities of LAT- and antigen-positive neurons within ganglia support the hypothesis that LAT-positive neurons were the neurons from which HSV was reactivated.

Original languageEnglish (US)
Pages (from-to)197-204
Number of pages8
JournalYale Journal of Biology and Medicine
Volume62
Issue number2
StatePublished - Jan 1 1989

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Herpes Simplex
Ganglia
Neurons
Viruses
Simplexvirus
Infection
Antigens
Virus Latency
Colchicine
Spinal Ganglia
In Situ Hybridization

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "Dorsal root ganglion neurons, which express herpes simplex virus (HSV) latency-associated transcript (LAT) during experimental latent infection, were investigated by in situ hybridization. The number of LAT-positive neurons was determined by examination of ganglion serial sections. In other latently infected mice, the number of ganglion neurons that reactivated HSV antigen after explant culture was determined in serial sections. LAG was detected in 100 percent of ganglia, with an average of 19.5 LAT-positive neurons per ganglion. After explant culture of latently infected ganglia (in the presence of colchicine to decrease spread of reactivated virus), HSV antigen was detected in 94 percent of ganglia, with an average of 13.1 positive neurons in the antigen-positive ganglia. The similar quantities of LAT- and antigen-positive neurons within ganglia support the hypothesis that LAT-positive neurons were the neurons from which HSV was reactivated.",
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Herpes simplex latent infection : Quantitation of latency-associated transcript-positive neurons and reactivable neurons. / Tenser, Richard; Edris, W. A.; Hay, K. A.

In: Yale Journal of Biology and Medicine, Vol. 62, No. 2, 01.01.1989, p. 197-204.

Research output: Contribution to journalArticle

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