Herpes simplex virus 1 pUL34 plays a critical role in cell-to-cell spread of virus in addition to its role in virus replication

Alison C. Haugo, Moriah Louise Szpara, Lance Parsons, Lynn W. Enquist, Richard J. Roller

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Herpes simplex virus (HSV) pUL34 plays a critical role in virus replication by mediating egress of nucleocapsids from the infected cell nucleus. We have identified a mutation in pUL34 (Y68A) that produces a major defect in virus replication and impaired nuclear egress but also profoundly inhibits cell-to-cell spread and trafficking of gE. Virion release to the extracellular medium is not affected by the Y68A mutation, indicating that the mutation specifically inhibits cell-to-cell spread. We isolated extragenic suppressors of the Y68A plaque formation defect and mapped them by a combination of high-throughput Illumina sequencing and PCR-based screening. We found that suppression is highly correlated with a nonsense mutation in the US9 gene, which plays a critical role in cell-to-cell spread of HSV-1 in neurons. The US9 mutation alone is not sufficient to suppress the Y68A spread phenotype, indicating a likely role for multiple viral factors.

Original languageEnglish (US)
Pages (from-to)7203-7215
Number of pages13
JournalJournal of virology
Volume85
Issue number14
DOIs
StatePublished - Jul 1 2011

Fingerprint

Human herpesvirus 1
Human Herpesvirus 1
Virus Replication
virus replication
Viruses
viruses
mutation
Mutation
cells
nonsense mutation
herpes simplex
Nucleocapsid
nucleocapsid
Nonsense Codon
Simplexvirus
cell nucleus
Cell Nucleus
virion
Virion
neurons

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Haugo, Alison C. ; Szpara, Moriah Louise ; Parsons, Lance ; Enquist, Lynn W. ; Roller, Richard J. / Herpes simplex virus 1 pUL34 plays a critical role in cell-to-cell spread of virus in addition to its role in virus replication. In: Journal of virology. 2011 ; Vol. 85, No. 14. pp. 7203-7215.
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Herpes simplex virus 1 pUL34 plays a critical role in cell-to-cell spread of virus in addition to its role in virus replication. / Haugo, Alison C.; Szpara, Moriah Louise; Parsons, Lance; Enquist, Lynn W.; Roller, Richard J.

In: Journal of virology, Vol. 85, No. 14, 01.07.2011, p. 7203-7215.

Research output: Contribution to journalArticle

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