Heterologous expression of maize (Zea mays L.) Mir1 cysteine proteinase in eukaryotic and prokaryotic expression systems

T. Pechan, P. W.K. Ma, D. S. Luthe

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Several heterologous expression systems were tested for their ability to express a unique maize cysteine proteinase Mir1. A baculovirus-based expression system using Trichoplusia ni larvae as host resulted in the expression of Mir1 that was correctly processed and exhibited proteinase activity. Expression in Escherichia coli resulted in accumulation of Mir1, but it had limited solubility and enzymatic activity. Large quantities of Mir1 were produced when Pichia pastoris was used as the host, but the enzyme was insoluble and inactive.

Original languageEnglish (US)
Pages (from-to)134-141
Number of pages8
JournalProtein Expression and Purification
Volume34
Issue number1
DOIs
StatePublished - Mar 2004

All Science Journal Classification (ASJC) codes

  • Biotechnology

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