Heterotrophic and mixotrophic nanoflagellates in a mesotrophic lake: Abundance and grazing impacts across season and depth

Sarah De Vaul Princiotta, Robert W. Sanders

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Nanoflagellates are recognized as fundamental members of the planktonic microbial food web due to their contribution to photosynthetic fixation of carbon and phagotrophic ingestion of bacteria. Although the presence, and often dominance, of mixotrophic nutrition among phytoflagellates has been well documented within a variety of aquatic ecosystems and in a range of taxa, few studies have assessed the abundance and grazing impact of mixotrophs in comparison to their photosynthetic and heterotrophic counterparts across seasons at multiple depths. Abundance and, as appropriate, bacterivory of phototrophic, heterotrophic, and mixotrophic nanoflagellates were quantified at three depths over a 15-month period in Lake Lacawac (Pocono Mountains, Pennsylvania, U.S.A.) via microscopic analysis of ingested bacterial surrogates. The absolute and relative abundances of all nanoflagellate trophic groups varied with season and depth, likely as a result of differences in physiological tolerance. Whereas the abundance of phototrophic and heterotrophic nanoflagellates varied with depth in both the presence and absence of thermal stratification, mixotrophic protists were evenly distributed throughout the water column during mixis. The abundances of phototrophic and mixotrophic protists were significantly correlated, but only in surface waters during periods of thermal stratification. Grazing rate and consequent impact by bacterivorous nanoflagellates varied with depth across season, particularly during thermal stratification. Mixotrophic bacterivory exceeded that of heterotrophs in the epi- and metalimnion during stratification, removing an estimated maximum of 80% of the bacterial standing stock d−1. The relative contribution of heterotrophic nanoflagellates to community bacterivory increased with depth, even during mixis, and dominated the grazing impact in the hypolimnion.

Original languageEnglish (US)
Pages (from-to)632-644
Number of pages13
JournalLimnology and Oceanography
Volume62
Issue number2
DOIs
StatePublished - Mar 1 2017

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grazing
bacterivory
lakes
lake
stratification
temperature profiles
protist
epilimnion
heterotrophs
hypolimnion
aquatic ecosystem
fixation
food webs
food web
nutrition
relative abundance
surface water
tolerance
water column
ingestion

All Science Journal Classification (ASJC) codes

  • Oceanography
  • Aquatic Science

Cite this

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abstract = "Nanoflagellates are recognized as fundamental members of the planktonic microbial food web due to their contribution to photosynthetic fixation of carbon and phagotrophic ingestion of bacteria. Although the presence, and often dominance, of mixotrophic nutrition among phytoflagellates has been well documented within a variety of aquatic ecosystems and in a range of taxa, few studies have assessed the abundance and grazing impact of mixotrophs in comparison to their photosynthetic and heterotrophic counterparts across seasons at multiple depths. Abundance and, as appropriate, bacterivory of phototrophic, heterotrophic, and mixotrophic nanoflagellates were quantified at three depths over a 15-month period in Lake Lacawac (Pocono Mountains, Pennsylvania, U.S.A.) via microscopic analysis of ingested bacterial surrogates. The absolute and relative abundances of all nanoflagellate trophic groups varied with season and depth, likely as a result of differences in physiological tolerance. Whereas the abundance of phototrophic and heterotrophic nanoflagellates varied with depth in both the presence and absence of thermal stratification, mixotrophic protists were evenly distributed throughout the water column during mixis. The abundances of phototrophic and mixotrophic protists were significantly correlated, but only in surface waters during periods of thermal stratification. Grazing rate and consequent impact by bacterivorous nanoflagellates varied with depth across season, particularly during thermal stratification. Mixotrophic bacterivory exceeded that of heterotrophs in the epi- and metalimnion during stratification, removing an estimated maximum of 80{\%} of the bacterial standing stock d−1. The relative contribution of heterotrophic nanoflagellates to community bacterivory increased with depth, even during mixis, and dominated the grazing impact in the hypolimnion.",
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Heterotrophic and mixotrophic nanoflagellates in a mesotrophic lake : Abundance and grazing impacts across season and depth. / Princiotta, Sarah De Vaul; Sanders, Robert W.

In: Limnology and Oceanography, Vol. 62, No. 2, 01.03.2017, p. 632-644.

Research output: Contribution to journalArticle

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