High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase

Kristin A. Eckert, Thomas A. Kunkel

Research output: Contribution to journalArticlepeer-review

300 Scopus citations

Abstract

We demonstrate that despite lacking a 3' - 5' proofreading exonuclease, the Thermus aquaticus (Taq) DNA polymerase can catalyze highly accurate DNA synthesis in vitro. Under defined reaction conditions, the error rate per nucleotide polymerized at 70°C can be as low as 10-5 for base substitution errors and 10-6 for frameshift errors. The frequency of mutations produced during a single round of DNA synthesis of the lac Zα gene by Taq polymerase responds to changes in dNTP concentration, pH, and the concentration of MgCI2 relative to the total concentration of deoxynucleotide triphosphates present in the reaction. Both base substitution and frameshift error rates of < 1/100, 000 were observed at pH 5-6 (70°C) or when MgCI2 and deoxynucleotide triphosphates were present at equimolar concentrations. These high fidelity reaction conditions for DNA synthesis by the Taq polymerase may be useful for specialized uses of DNA amplified by the polymerase chain reaction.

Original languageEnglish (US)
Pages (from-to)3739-3744
Number of pages6
JournalNucleic acids research
Volume18
Issue number13
DOIs
StatePublished - Jul 11 1990

All Science Journal Classification (ASJC) codes

  • Genetics

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