High-frequency callus induction and plant regeneration in Tripsacum dactyloides (L.)

Sairam V. Rudrabhatla, C. Wilber, J. Franklin, B. Smith, J. Bazil, R. Hassel, D. Whaling, K. Frutiger, C. A. Blakey, R. Vierling, S. L. Goldman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A protocol for high-frequency callus, somatic embryogenesis, and plant regeneration for Tripsacum is described. Plants were regenerated from complete shoot meristems (3-4mm) via organogenesis and embryogenesis. In organogenesis, the shoot meristems were cultured directly on a high cytokinin medium comprising 5-10mgl-1 (22.2-44.4μM) 6-benzyladenine (BA). The number of multiple shoots varied from six to eight from each meristem. The time required for production of plants from organogenesis was rapid (4-6wk). In contrast, callus was induced on an auxin medium and continuously cultured on an auxin medium for production of somatic embryos. Prolific callus with numerous somatic embryos developed within 3-4wk when cultured on an auxin medium containing 5mgl-1 (22.6μM) 2,4-dichlorophenoxyacetic acid (2,4-D). The number of shoots induced varied from two to five per callus. Regardless of the cultivars used, the frequency of callus induction and plant regeneration was between 48% and 94%. The seed germination procedures also were modified and resulted in a maximum of 60-80% seed germination. Finally, the rate of T-DNA transfer to complete shoot meristems of Tripsacum was high on the auxin medium and was independent of whether super-virulent strains of Agrobacterium were used or not.

Original languageEnglish (US)
Pages (from-to)435-440
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume38
Issue number5
DOIs
StatePublished - Sep 1 2002

Fingerprint

Tripsacum dactyloides
Bony Callus
Indoleacetic Acids
Meristem
Regeneration
callus
auxins
shoot meristems
organogenesis
Tripsacum
2,4-Dichlorophenoxyacetic Acid
N-benzyladenine
Organogenesis
Germination
somatic embryos
2,4-D
Plant Organogenesis
Seeds
Plant Somatic Embryogenesis Techniques
seed germination

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Plant Science

Cite this

Rudrabhatla, Sairam V. ; Wilber, C. ; Franklin, J. ; Smith, B. ; Bazil, J. ; Hassel, R. ; Whaling, D. ; Frutiger, K. ; Blakey, C. A. ; Vierling, R. ; Goldman, S. L. / High-frequency callus induction and plant regeneration in Tripsacum dactyloides (L.). In: In Vitro Cellular and Developmental Biology - Plant. 2002 ; Vol. 38, No. 5. pp. 435-440.
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Rudrabhatla, SV, Wilber, C, Franklin, J, Smith, B, Bazil, J, Hassel, R, Whaling, D, Frutiger, K, Blakey, CA, Vierling, R & Goldman, SL 2002, 'High-frequency callus induction and plant regeneration in Tripsacum dactyloides (L.)', In Vitro Cellular and Developmental Biology - Plant, vol. 38, no. 5, pp. 435-440. https://doi.org/10.1079/IVP2002314

High-frequency callus induction and plant regeneration in Tripsacum dactyloides (L.). / Rudrabhatla, Sairam V.; Wilber, C.; Franklin, J.; Smith, B.; Bazil, J.; Hassel, R.; Whaling, D.; Frutiger, K.; Blakey, C. A.; Vierling, R.; Goldman, S. L.

In: In Vitro Cellular and Developmental Biology - Plant, Vol. 38, No. 5, 01.09.2002, p. 435-440.

Research output: Contribution to journalArticle

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AU - Wilber, C.

AU - Franklin, J.

AU - Smith, B.

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AU - Hassel, R.

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