High Level of Neutrophil Extracellular Traps Correlates with Poor Prognosis of Severe Influenza A Infection

Liuluan Zhu, Lu Liu, Yue Zhang, Lin Pu, Jingyuan Liu, Xingwang Li, Zhihai Chen, Yu Hao, Beibei Wang, Junyan Han, Guoli Li, Shuntao Liang, Haofeng Xiong, Hong Zheng, Ang Li, Jianqing Xu, Hui Zeng

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background. Most patients with severe infection with influenza A virus (IAV) progress to acute respiratory distress syndrome and even multiple organ dysfunction syndrome (MODS). Neutrophil extracellular traps (NETs) can be induced by pathogens and are responsible for immune tissue damage. We conducted a prospective study on the production and effects of NETs in H7N9 and H1N1 patients. Methods. We investigated NET production in plasma and supernatant of cultured neutrophils by measuring cell-free deoxyribonucleic acid (DNA) and myeloperoxidase (MPO)-DNA complexes with PicoGreen dye and enzyme-linked immunosorbent assay methods, respectively. We also observed NET structure by immunofluorescence staining. Results. We found that patients with severe influenza showed elevated plasma NET level on the day of admission. Neutrophils from these patients showed higher capacity to release MPO-DNA complex in response to interleukin-8 or lipopolysaccharide stimulation. We also found that NETs from H7N9 and H1N1 patients increased the permeability of alveolar epithelial cells, and, consequently, NET production was positively correlated with acute physiology and chronic health evaluation (APACHE) II score and MODS. Conclusions. These data indicate that high level of NETs contributes to lung injury and is correlated with severity of disease. Thus, NETs might be a key factor to predict the poor prognosis in IAV patients.

Original languageEnglish (US)
Pages (from-to)428-437
Number of pages10
JournalJournal of Infectious Diseases
Volume217
Issue number3
DOIs
StatePublished - Jan 17 2018

Fingerprint

Human Influenza
Infection
Multiple Organ Failure
Influenza A virus
Peroxidase
DNA
Neutrophils
Alveolar Epithelial Cells
Extracellular Traps
APACHE
Adult Respiratory Distress Syndrome
Lung Injury
Interleukin-8
Fluorescent Antibody Technique
Lipopolysaccharides
Permeability
Coloring Agents
Enzyme-Linked Immunosorbent Assay
Prospective Studies
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Zhu, Liuluan ; Liu, Lu ; Zhang, Yue ; Pu, Lin ; Liu, Jingyuan ; Li, Xingwang ; Chen, Zhihai ; Hao, Yu ; Wang, Beibei ; Han, Junyan ; Li, Guoli ; Liang, Shuntao ; Xiong, Haofeng ; Zheng, Hong ; Li, Ang ; Xu, Jianqing ; Zeng, Hui. / High Level of Neutrophil Extracellular Traps Correlates with Poor Prognosis of Severe Influenza A Infection. In: Journal of Infectious Diseases. 2018 ; Vol. 217, No. 3. pp. 428-437.
@article{57e24bbbf361433ba50d4fea5c0f5ea0,
title = "High Level of Neutrophil Extracellular Traps Correlates with Poor Prognosis of Severe Influenza A Infection",
abstract = "Background. Most patients with severe infection with influenza A virus (IAV) progress to acute respiratory distress syndrome and even multiple organ dysfunction syndrome (MODS). Neutrophil extracellular traps (NETs) can be induced by pathogens and are responsible for immune tissue damage. We conducted a prospective study on the production and effects of NETs in H7N9 and H1N1 patients. Methods. We investigated NET production in plasma and supernatant of cultured neutrophils by measuring cell-free deoxyribonucleic acid (DNA) and myeloperoxidase (MPO)-DNA complexes with PicoGreen dye and enzyme-linked immunosorbent assay methods, respectively. We also observed NET structure by immunofluorescence staining. Results. We found that patients with severe influenza showed elevated plasma NET level on the day of admission. Neutrophils from these patients showed higher capacity to release MPO-DNA complex in response to interleukin-8 or lipopolysaccharide stimulation. We also found that NETs from H7N9 and H1N1 patients increased the permeability of alveolar epithelial cells, and, consequently, NET production was positively correlated with acute physiology and chronic health evaluation (APACHE) II score and MODS. Conclusions. These data indicate that high level of NETs contributes to lung injury and is correlated with severity of disease. Thus, NETs might be a key factor to predict the poor prognosis in IAV patients.",
author = "Liuluan Zhu and Lu Liu and Yue Zhang and Lin Pu and Jingyuan Liu and Xingwang Li and Zhihai Chen and Yu Hao and Beibei Wang and Junyan Han and Guoli Li and Shuntao Liang and Haofeng Xiong and Hong Zheng and Ang Li and Jianqing Xu and Hui Zeng",
year = "2018",
month = "1",
day = "17",
doi = "10.1093/infdis/jix475",
language = "English (US)",
volume = "217",
pages = "428--437",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "3",

}

Zhu, L, Liu, L, Zhang, Y, Pu, L, Liu, J, Li, X, Chen, Z, Hao, Y, Wang, B, Han, J, Li, G, Liang, S, Xiong, H, Zheng, H, Li, A, Xu, J & Zeng, H 2018, 'High Level of Neutrophil Extracellular Traps Correlates with Poor Prognosis of Severe Influenza A Infection', Journal of Infectious Diseases, vol. 217, no. 3, pp. 428-437. https://doi.org/10.1093/infdis/jix475

High Level of Neutrophil Extracellular Traps Correlates with Poor Prognosis of Severe Influenza A Infection. / Zhu, Liuluan; Liu, Lu; Zhang, Yue; Pu, Lin; Liu, Jingyuan; Li, Xingwang; Chen, Zhihai; Hao, Yu; Wang, Beibei; Han, Junyan; Li, Guoli; Liang, Shuntao; Xiong, Haofeng; Zheng, Hong; Li, Ang; Xu, Jianqing; Zeng, Hui.

In: Journal of Infectious Diseases, Vol. 217, No. 3, 17.01.2018, p. 428-437.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High Level of Neutrophil Extracellular Traps Correlates with Poor Prognosis of Severe Influenza A Infection

AU - Zhu, Liuluan

AU - Liu, Lu

AU - Zhang, Yue

AU - Pu, Lin

AU - Liu, Jingyuan

AU - Li, Xingwang

AU - Chen, Zhihai

AU - Hao, Yu

AU - Wang, Beibei

AU - Han, Junyan

AU - Li, Guoli

AU - Liang, Shuntao

AU - Xiong, Haofeng

AU - Zheng, Hong

AU - Li, Ang

AU - Xu, Jianqing

AU - Zeng, Hui

PY - 2018/1/17

Y1 - 2018/1/17

N2 - Background. Most patients with severe infection with influenza A virus (IAV) progress to acute respiratory distress syndrome and even multiple organ dysfunction syndrome (MODS). Neutrophil extracellular traps (NETs) can be induced by pathogens and are responsible for immune tissue damage. We conducted a prospective study on the production and effects of NETs in H7N9 and H1N1 patients. Methods. We investigated NET production in plasma and supernatant of cultured neutrophils by measuring cell-free deoxyribonucleic acid (DNA) and myeloperoxidase (MPO)-DNA complexes with PicoGreen dye and enzyme-linked immunosorbent assay methods, respectively. We also observed NET structure by immunofluorescence staining. Results. We found that patients with severe influenza showed elevated plasma NET level on the day of admission. Neutrophils from these patients showed higher capacity to release MPO-DNA complex in response to interleukin-8 or lipopolysaccharide stimulation. We also found that NETs from H7N9 and H1N1 patients increased the permeability of alveolar epithelial cells, and, consequently, NET production was positively correlated with acute physiology and chronic health evaluation (APACHE) II score and MODS. Conclusions. These data indicate that high level of NETs contributes to lung injury and is correlated with severity of disease. Thus, NETs might be a key factor to predict the poor prognosis in IAV patients.

AB - Background. Most patients with severe infection with influenza A virus (IAV) progress to acute respiratory distress syndrome and even multiple organ dysfunction syndrome (MODS). Neutrophil extracellular traps (NETs) can be induced by pathogens and are responsible for immune tissue damage. We conducted a prospective study on the production and effects of NETs in H7N9 and H1N1 patients. Methods. We investigated NET production in plasma and supernatant of cultured neutrophils by measuring cell-free deoxyribonucleic acid (DNA) and myeloperoxidase (MPO)-DNA complexes with PicoGreen dye and enzyme-linked immunosorbent assay methods, respectively. We also observed NET structure by immunofluorescence staining. Results. We found that patients with severe influenza showed elevated plasma NET level on the day of admission. Neutrophils from these patients showed higher capacity to release MPO-DNA complex in response to interleukin-8 or lipopolysaccharide stimulation. We also found that NETs from H7N9 and H1N1 patients increased the permeability of alveolar epithelial cells, and, consequently, NET production was positively correlated with acute physiology and chronic health evaluation (APACHE) II score and MODS. Conclusions. These data indicate that high level of NETs contributes to lung injury and is correlated with severity of disease. Thus, NETs might be a key factor to predict the poor prognosis in IAV patients.

UR - http://www.scopus.com/inward/record.url?scp=85041178597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041178597&partnerID=8YFLogxK

U2 - 10.1093/infdis/jix475

DO - 10.1093/infdis/jix475

M3 - Article

C2 - 29325098

AN - SCOPUS:85041178597

VL - 217

SP - 428

EP - 437

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 3

ER -