High-oleic canola oil consumption enriches LDL particle cholesteryl oleate content and reduces LDL proteoglycan binding in humans

Peter J.H. Jones, Dylan S. MacKay, Vijitha K. Senanayake, Shuaihua Pu, David J.A. Jenkins, Philip W. Connelly, Benoît Lamarche, Patrick Couture, Penny M. Kris-Etherton, Sheila G. West, Xiaoran Liu, Jennifer A. Fleming, Roy R. Hantgan, Lawrence L. Rudel

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Oleic acid consumption is considered cardio-protective according to studies conducted examining effects of the Mediterranean diet. However, animal models have shown that oleic acid consumption increases LDL particle cholesteryl oleate content which is associated with increased LDL-proteoglycan binding and atherosclerosis. The objective was to examine effects of varying oleic, linoleic and docosahexaenoic acid consumption on human LDL-proteoglycan binding in a non-random subset of the Canola Oil Multi-center Intervention Trial (COMIT) participants. COMIT employed a randomized, double-blind, five-period, cross-over trial design. Three of the treatment oil diets: 1) a blend of corn/safflower oil (25:75); 2) high oleic canola oil; and 3) DHA-enriched high oleic canola oil were selected for analysis of LDL-proteoglycan binding in 50 participants exhibiting good compliance. LDL particles were isolated from frozen plasma by gel filtration chromatography and LDL cholesteryl esters quantified by mass-spectrometry. LDL-proteoglycan binding was assessed using surface plasmon resonance. LDL particle cholesterol ester fatty acid composition was sensitive to the treatment fatty acid compositions, with the main fatty acids in the treatments increasing in the LDL cholesterol esters. The corn/safflower oil and high-oleic canola oil diets lowered LDL-proteoglycan binding relative to their baseline values ( p=0.0005 and p=0.0012, respectively). At endpoint, high-oleic canola oil feeding resulted in lower LDL-proteoglycan binding than corn/safflower oil ( p=0.0243) and DHA-enriched high oleic canola oil ( p=0.0249), although high-oleic canola oil had the lowest binding at baseline ( p=0.0344). Our findings suggest that high-oleic canola oil consumption in humans increases cholesteryl oleate percentage in LDL, but in a manner not associated with a rise in LDL-proteoglycan binding.

Original languageEnglish (US)
Pages (from-to)231-238
Number of pages8
JournalAtherosclerosis
Volume238
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Proteoglycans
Safflower Oil
Corn Oil
Cholesterol Esters
Fatty Acids
Oleic Acid
LDL Cholesterol
Cross-Over Studies
Diet
Mediterranean Diet
canola oil
cholesteryl oleate
oxidized low density lipoprotein
Surface Plasmon Resonance
Linoleic Acid
Gel Chromatography
Mass Spectrometry
Atherosclerosis
Oils
Therapeutics

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Jones, P. J. H., MacKay, D. S., Senanayake, V. K., Pu, S., Jenkins, D. J. A., Connelly, P. W., ... Rudel, L. L. (2015). High-oleic canola oil consumption enriches LDL particle cholesteryl oleate content and reduces LDL proteoglycan binding in humans. Atherosclerosis, 238(2), 231-238. https://doi.org/10.1016/j.atherosclerosis.2014.12.010
Jones, Peter J.H. ; MacKay, Dylan S. ; Senanayake, Vijitha K. ; Pu, Shuaihua ; Jenkins, David J.A. ; Connelly, Philip W. ; Lamarche, Benoît ; Couture, Patrick ; Kris-Etherton, Penny M. ; West, Sheila G. ; Liu, Xiaoran ; Fleming, Jennifer A. ; Hantgan, Roy R. ; Rudel, Lawrence L. / High-oleic canola oil consumption enriches LDL particle cholesteryl oleate content and reduces LDL proteoglycan binding in humans. In: Atherosclerosis. 2015 ; Vol. 238, No. 2. pp. 231-238.
@article{44ef01f48e75491fb9c25771915595d5,
title = "High-oleic canola oil consumption enriches LDL particle cholesteryl oleate content and reduces LDL proteoglycan binding in humans",
abstract = "Oleic acid consumption is considered cardio-protective according to studies conducted examining effects of the Mediterranean diet. However, animal models have shown that oleic acid consumption increases LDL particle cholesteryl oleate content which is associated with increased LDL-proteoglycan binding and atherosclerosis. The objective was to examine effects of varying oleic, linoleic and docosahexaenoic acid consumption on human LDL-proteoglycan binding in a non-random subset of the Canola Oil Multi-center Intervention Trial (COMIT) participants. COMIT employed a randomized, double-blind, five-period, cross-over trial design. Three of the treatment oil diets: 1) a blend of corn/safflower oil (25:75); 2) high oleic canola oil; and 3) DHA-enriched high oleic canola oil were selected for analysis of LDL-proteoglycan binding in 50 participants exhibiting good compliance. LDL particles were isolated from frozen plasma by gel filtration chromatography and LDL cholesteryl esters quantified by mass-spectrometry. LDL-proteoglycan binding was assessed using surface plasmon resonance. LDL particle cholesterol ester fatty acid composition was sensitive to the treatment fatty acid compositions, with the main fatty acids in the treatments increasing in the LDL cholesterol esters. The corn/safflower oil and high-oleic canola oil diets lowered LDL-proteoglycan binding relative to their baseline values ( p=0.0005 and p=0.0012, respectively). At endpoint, high-oleic canola oil feeding resulted in lower LDL-proteoglycan binding than corn/safflower oil ( p=0.0243) and DHA-enriched high oleic canola oil ( p=0.0249), although high-oleic canola oil had the lowest binding at baseline ( p=0.0344). Our findings suggest that high-oleic canola oil consumption in humans increases cholesteryl oleate percentage in LDL, but in a manner not associated with a rise in LDL-proteoglycan binding.",
author = "Jones, {Peter J.H.} and MacKay, {Dylan S.} and Senanayake, {Vijitha K.} and Shuaihua Pu and Jenkins, {David J.A.} and Connelly, {Philip W.} and Beno{\^i}t Lamarche and Patrick Couture and Kris-Etherton, {Penny M.} and West, {Sheila G.} and Xiaoran Liu and Fleming, {Jennifer A.} and Hantgan, {Roy R.} and Rudel, {Lawrence L.}",
year = "2015",
month = "2",
day = "1",
doi = "10.1016/j.atherosclerosis.2014.12.010",
language = "English (US)",
volume = "238",
pages = "231--238",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

Jones, PJH, MacKay, DS, Senanayake, VK, Pu, S, Jenkins, DJA, Connelly, PW, Lamarche, B, Couture, P, Kris-Etherton, PM, West, SG, Liu, X, Fleming, JA, Hantgan, RR & Rudel, LL 2015, 'High-oleic canola oil consumption enriches LDL particle cholesteryl oleate content and reduces LDL proteoglycan binding in humans', Atherosclerosis, vol. 238, no. 2, pp. 231-238. https://doi.org/10.1016/j.atherosclerosis.2014.12.010

High-oleic canola oil consumption enriches LDL particle cholesteryl oleate content and reduces LDL proteoglycan binding in humans. / Jones, Peter J.H.; MacKay, Dylan S.; Senanayake, Vijitha K.; Pu, Shuaihua; Jenkins, David J.A.; Connelly, Philip W.; Lamarche, Benoît; Couture, Patrick; Kris-Etherton, Penny M.; West, Sheila G.; Liu, Xiaoran; Fleming, Jennifer A.; Hantgan, Roy R.; Rudel, Lawrence L.

In: Atherosclerosis, Vol. 238, No. 2, 01.02.2015, p. 231-238.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High-oleic canola oil consumption enriches LDL particle cholesteryl oleate content and reduces LDL proteoglycan binding in humans

AU - Jones, Peter J.H.

AU - MacKay, Dylan S.

AU - Senanayake, Vijitha K.

AU - Pu, Shuaihua

AU - Jenkins, David J.A.

AU - Connelly, Philip W.

AU - Lamarche, Benoît

AU - Couture, Patrick

AU - Kris-Etherton, Penny M.

AU - West, Sheila G.

AU - Liu, Xiaoran

AU - Fleming, Jennifer A.

AU - Hantgan, Roy R.

AU - Rudel, Lawrence L.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Oleic acid consumption is considered cardio-protective according to studies conducted examining effects of the Mediterranean diet. However, animal models have shown that oleic acid consumption increases LDL particle cholesteryl oleate content which is associated with increased LDL-proteoglycan binding and atherosclerosis. The objective was to examine effects of varying oleic, linoleic and docosahexaenoic acid consumption on human LDL-proteoglycan binding in a non-random subset of the Canola Oil Multi-center Intervention Trial (COMIT) participants. COMIT employed a randomized, double-blind, five-period, cross-over trial design. Three of the treatment oil diets: 1) a blend of corn/safflower oil (25:75); 2) high oleic canola oil; and 3) DHA-enriched high oleic canola oil were selected for analysis of LDL-proteoglycan binding in 50 participants exhibiting good compliance. LDL particles were isolated from frozen plasma by gel filtration chromatography and LDL cholesteryl esters quantified by mass-spectrometry. LDL-proteoglycan binding was assessed using surface plasmon resonance. LDL particle cholesterol ester fatty acid composition was sensitive to the treatment fatty acid compositions, with the main fatty acids in the treatments increasing in the LDL cholesterol esters. The corn/safflower oil and high-oleic canola oil diets lowered LDL-proteoglycan binding relative to their baseline values ( p=0.0005 and p=0.0012, respectively). At endpoint, high-oleic canola oil feeding resulted in lower LDL-proteoglycan binding than corn/safflower oil ( p=0.0243) and DHA-enriched high oleic canola oil ( p=0.0249), although high-oleic canola oil had the lowest binding at baseline ( p=0.0344). Our findings suggest that high-oleic canola oil consumption in humans increases cholesteryl oleate percentage in LDL, but in a manner not associated with a rise in LDL-proteoglycan binding.

AB - Oleic acid consumption is considered cardio-protective according to studies conducted examining effects of the Mediterranean diet. However, animal models have shown that oleic acid consumption increases LDL particle cholesteryl oleate content which is associated with increased LDL-proteoglycan binding and atherosclerosis. The objective was to examine effects of varying oleic, linoleic and docosahexaenoic acid consumption on human LDL-proteoglycan binding in a non-random subset of the Canola Oil Multi-center Intervention Trial (COMIT) participants. COMIT employed a randomized, double-blind, five-period, cross-over trial design. Three of the treatment oil diets: 1) a blend of corn/safflower oil (25:75); 2) high oleic canola oil; and 3) DHA-enriched high oleic canola oil were selected for analysis of LDL-proteoglycan binding in 50 participants exhibiting good compliance. LDL particles were isolated from frozen plasma by gel filtration chromatography and LDL cholesteryl esters quantified by mass-spectrometry. LDL-proteoglycan binding was assessed using surface plasmon resonance. LDL particle cholesterol ester fatty acid composition was sensitive to the treatment fatty acid compositions, with the main fatty acids in the treatments increasing in the LDL cholesterol esters. The corn/safflower oil and high-oleic canola oil diets lowered LDL-proteoglycan binding relative to their baseline values ( p=0.0005 and p=0.0012, respectively). At endpoint, high-oleic canola oil feeding resulted in lower LDL-proteoglycan binding than corn/safflower oil ( p=0.0243) and DHA-enriched high oleic canola oil ( p=0.0249), although high-oleic canola oil had the lowest binding at baseline ( p=0.0344). Our findings suggest that high-oleic canola oil consumption in humans increases cholesteryl oleate percentage in LDL, but in a manner not associated with a rise in LDL-proteoglycan binding.

UR - http://www.scopus.com/inward/record.url?scp=84919798233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84919798233&partnerID=8YFLogxK

U2 - 10.1016/j.atherosclerosis.2014.12.010

DO - 10.1016/j.atherosclerosis.2014.12.010

M3 - Article

C2 - 25528432

AN - SCOPUS:84919798233

VL - 238

SP - 231

EP - 238

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 2

ER -