High-performance size-exclusion chromatography (HPSEC) and fluorophore-assisted carbohydrate electrophoresis (FACE) to describe the chain-length distribution of debranched starch

Yuan Yao, Mark J. Guiltinan, Donald B. Thompson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Chain-length (CL) distribution is an important feature of the 'fine structure' of starch molecules, which are comprised of amylose and amylopectin. The objective of the present work was to combine data for two methods to achieve a more comprehensive data set that would allow a fuller comparison of the CL distribution for different starches. Both high-performance size-exclusion chromatography (HPSEC) and fluorophore-assisted carbohydrate electrophoresis (FACE) were carried out on endosperm starch isolated from five maize genotypes. For the CL distribution in the range DP ≤ 50, data in the HPSEC chromatogram were transformed to the form of a FACE electrophoregram, in which the x-axis is DP and the y-axis is the number of chains. The two sets of data in this region were shown to be similar. We conclude that the data sets from HPSEC and FACE may be considered together to describe the CL distribution more completely than for either method alone. We further note that for DP 6-50, data from HPSEC may be transformed to allow a similar presentation as for that obtained by FACE, such that FACE analysis might not be required for comparison of CL distribution of different starches.

Original languageEnglish (US)
Pages (from-to)701-710
Number of pages10
JournalCarbohydrate Research
Volume340
Issue number4
DOIs
StatePublished - Mar 21 2005

Fingerprint

Size exclusion chromatography
Fluorophores
Electrophoresis
Chain length
Starch
Gel Chromatography
Carbohydrates
Amylopectin
Amylose
Endosperm
Zea mays
Genotype
Molecules
Datasets

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

@article{f821fd2e8f6a4685b1ee847ab2110ae6,
title = "High-performance size-exclusion chromatography (HPSEC) and fluorophore-assisted carbohydrate electrophoresis (FACE) to describe the chain-length distribution of debranched starch",
abstract = "Chain-length (CL) distribution is an important feature of the 'fine structure' of starch molecules, which are comprised of amylose and amylopectin. The objective of the present work was to combine data for two methods to achieve a more comprehensive data set that would allow a fuller comparison of the CL distribution for different starches. Both high-performance size-exclusion chromatography (HPSEC) and fluorophore-assisted carbohydrate electrophoresis (FACE) were carried out on endosperm starch isolated from five maize genotypes. For the CL distribution in the range DP ≤ 50, data in the HPSEC chromatogram were transformed to the form of a FACE electrophoregram, in which the x-axis is DP and the y-axis is the number of chains. The two sets of data in this region were shown to be similar. We conclude that the data sets from HPSEC and FACE may be considered together to describe the CL distribution more completely than for either method alone. We further note that for DP 6-50, data from HPSEC may be transformed to allow a similar presentation as for that obtained by FACE, such that FACE analysis might not be required for comparison of CL distribution of different starches.",
author = "Yuan Yao and Guiltinan, {Mark J.} and Thompson, {Donald B.}",
year = "2005",
month = "3",
day = "21",
doi = "10.1016/j.carres.2004.12.033",
language = "English (US)",
volume = "340",
pages = "701--710",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - High-performance size-exclusion chromatography (HPSEC) and fluorophore-assisted carbohydrate electrophoresis (FACE) to describe the chain-length distribution of debranched starch

AU - Yao, Yuan

AU - Guiltinan, Mark J.

AU - Thompson, Donald B.

PY - 2005/3/21

Y1 - 2005/3/21

N2 - Chain-length (CL) distribution is an important feature of the 'fine structure' of starch molecules, which are comprised of amylose and amylopectin. The objective of the present work was to combine data for two methods to achieve a more comprehensive data set that would allow a fuller comparison of the CL distribution for different starches. Both high-performance size-exclusion chromatography (HPSEC) and fluorophore-assisted carbohydrate electrophoresis (FACE) were carried out on endosperm starch isolated from five maize genotypes. For the CL distribution in the range DP ≤ 50, data in the HPSEC chromatogram were transformed to the form of a FACE electrophoregram, in which the x-axis is DP and the y-axis is the number of chains. The two sets of data in this region were shown to be similar. We conclude that the data sets from HPSEC and FACE may be considered together to describe the CL distribution more completely than for either method alone. We further note that for DP 6-50, data from HPSEC may be transformed to allow a similar presentation as for that obtained by FACE, such that FACE analysis might not be required for comparison of CL distribution of different starches.

AB - Chain-length (CL) distribution is an important feature of the 'fine structure' of starch molecules, which are comprised of amylose and amylopectin. The objective of the present work was to combine data for two methods to achieve a more comprehensive data set that would allow a fuller comparison of the CL distribution for different starches. Both high-performance size-exclusion chromatography (HPSEC) and fluorophore-assisted carbohydrate electrophoresis (FACE) were carried out on endosperm starch isolated from five maize genotypes. For the CL distribution in the range DP ≤ 50, data in the HPSEC chromatogram were transformed to the form of a FACE electrophoregram, in which the x-axis is DP and the y-axis is the number of chains. The two sets of data in this region were shown to be similar. We conclude that the data sets from HPSEC and FACE may be considered together to describe the CL distribution more completely than for either method alone. We further note that for DP 6-50, data from HPSEC may be transformed to allow a similar presentation as for that obtained by FACE, such that FACE analysis might not be required for comparison of CL distribution of different starches.

UR - http://www.scopus.com/inward/record.url?scp=13844313403&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844313403&partnerID=8YFLogxK

U2 - 10.1016/j.carres.2004.12.033

DO - 10.1016/j.carres.2004.12.033

M3 - Article

C2 - 15721343

AN - SCOPUS:13844313403

VL - 340

SP - 701

EP - 710

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

IS - 4

ER -