Abstract
We describe an analytical method to measure 2,3,7,8-tetra-chlorodlbenzo-p-dioxin (TCDD) and other TCDD Isomers In human serum; the method uses a highly specific cleanup procedure and hlgh-resolutlon gas chromatography/hlgh-res-olutlon mass spectrometry. The 2,3,7,8-TCDD Is quantified by the Isotope dilution technique with [13C12]-2,3,7,8-TCDD as the Internal standard. Other TCDD Isomers are estimated by using published relative response factors. The 1.25 part-per-quadrllllon (ppq) limit of detection for 200-g samples is more than adequate for determining 2,3,7,8-TCDD concentrations In human serum specimens. The method Is modified for analyzing 50-g and 10-g serum samples. Analytical accuracy is demonstrated by the results obtained in analyzing spiked samples. The method is shown to be unaffected by a number of potentially Interfering compounds. A series of quality control material Is used to verify system performance. For 200-g samples containing 25.8 ppq of native 2,3,7,8-TCDD, a coefficient of variation (CV) of 13.0% Is observed (n = 20). For 10-g samples from a pool fortified with 2,3,7,8-TCDD (1.9 parts per trillion, n = 22), a CV of 15.5% is observed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2000-2005 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 59 |
| Issue number | 15 |
| DOIs | |
| State | Published - Jan 1 1987 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
Cite this
}
High-Resolution Gas Chromatographic/High-Resolution Mass Spectrometric Analysis of Human Serum on a Whole-Weight and Lipid Basis for 2,3,7,8-Tetrachlorodibenzo-p-dioxin. / Patterson, Donald George Jr; Hampton, Larry; Lapeza, Chester R.; Belser, William T.; Green, Vaughn; Alexander, Louis; Needham, Larry L.
In: Analytical Chemistry, Vol. 59, No. 15, 01.01.1987, p. 2000-2005.Research output: Contribution to journal › Article
TY - JOUR
T1 - High-Resolution Gas Chromatographic/High-Resolution Mass Spectrometric Analysis of Human Serum on a Whole-Weight and Lipid Basis for 2,3,7,8-Tetrachlorodibenzo-p-dioxin
AU - Patterson, Donald George Jr
AU - Hampton, Larry
AU - Lapeza, Chester R.
AU - Belser, William T.
AU - Green, Vaughn
AU - Alexander, Louis
AU - Needham, Larry L.
PY - 1987/1/1
Y1 - 1987/1/1
N2 - We describe an analytical method to measure 2,3,7,8-tetra-chlorodlbenzo-p-dioxin (TCDD) and other TCDD Isomers In human serum; the method uses a highly specific cleanup procedure and hlgh-resolutlon gas chromatography/hlgh-res-olutlon mass spectrometry. The 2,3,7,8-TCDD Is quantified by the Isotope dilution technique with [13C12]-2,3,7,8-TCDD as the Internal standard. Other TCDD Isomers are estimated by using published relative response factors. The 1.25 part-per-quadrllllon (ppq) limit of detection for 200-g samples is more than adequate for determining 2,3,7,8-TCDD concentrations In human serum specimens. The method Is modified for analyzing 50-g and 10-g serum samples. Analytical accuracy is demonstrated by the results obtained in analyzing spiked samples. The method is shown to be unaffected by a number of potentially Interfering compounds. A series of quality control material Is used to verify system performance. For 200-g samples containing 25.8 ppq of native 2,3,7,8-TCDD, a coefficient of variation (CV) of 13.0% Is observed (n = 20). For 10-g samples from a pool fortified with 2,3,7,8-TCDD (1.9 parts per trillion, n = 22), a CV of 15.5% is observed.
AB - We describe an analytical method to measure 2,3,7,8-tetra-chlorodlbenzo-p-dioxin (TCDD) and other TCDD Isomers In human serum; the method uses a highly specific cleanup procedure and hlgh-resolutlon gas chromatography/hlgh-res-olutlon mass spectrometry. The 2,3,7,8-TCDD Is quantified by the Isotope dilution technique with [13C12]-2,3,7,8-TCDD as the Internal standard. Other TCDD Isomers are estimated by using published relative response factors. The 1.25 part-per-quadrllllon (ppq) limit of detection for 200-g samples is more than adequate for determining 2,3,7,8-TCDD concentrations In human serum specimens. The method Is modified for analyzing 50-g and 10-g serum samples. Analytical accuracy is demonstrated by the results obtained in analyzing spiked samples. The method is shown to be unaffected by a number of potentially Interfering compounds. A series of quality control material Is used to verify system performance. For 200-g samples containing 25.8 ppq of native 2,3,7,8-TCDD, a coefficient of variation (CV) of 13.0% Is observed (n = 20). For 10-g samples from a pool fortified with 2,3,7,8-TCDD (1.9 parts per trillion, n = 22), a CV of 15.5% is observed.
UR - http://www.scopus.com/inward/record.url?scp=0023395329&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023395329&partnerID=8YFLogxK
U2 - 10.1021/ac00142a023
DO - 10.1021/ac00142a023
M3 - Article
VL - 59
SP - 2000
EP - 2005
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 15
ER -