High-resolution genotyping of Streptococcus pyogenes serotype M1 isolates by fluorescent amplified-fragment length polymorphism analysis

Meeta Desai, Androulla Efstratiou, Robert George, John Stanley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have used fluorescent amplified-fragment length polymorphism (FAFLP) analysis to subtype clinical isolates of Streptococcus pyogenes serotype M1. Established typing methods define most M1 isolates as members of a clone that has a worldwide distribution and that is strongly associated with invasive diseases. FAFLP analysis simultaneously sampled 90 to 120 loci throughout the M1 genome. Its discriminatory power, precision, and reproducibility were compared with those of other molecular typing methods. Irrespective of disease symptomatology or geographic origin, the majority of the clinical M1 isolates shared a single ribotype, pulsed-field gel electrophoresis macrorestriction profile, and emm1 gene sequence. Nonetheless, among these isolates, FAFLP analysis could differentiate 17 distinct profiles, including seven multi-isolate groups. The FAFLP profiles of M1 isolates reproducibly exhibited between 1 and more than 20 amplified fragment differences. The high discriminatory power of genotyping by FAFLP analysis revealed genetic microheterogeneity and differentiated otherwise 'identical' M1 isolates as members of a clone complex.

Original languageEnglish (US)
Pages (from-to)1948-1952
Number of pages5
JournalJournal of clinical microbiology
Volume37
Issue number6
StatePublished - May 29 1999

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Amplified Fragment Length Polymorphism Analysis
Streptococcus pyogenes
Clone Cells
Ribotyping
Molecular Typing
Pulsed Field Gel Electrophoresis
Genome
Serogroup
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

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High-resolution genotyping of Streptococcus pyogenes serotype M1 isolates by fluorescent amplified-fragment length polymorphism analysis. / Desai, Meeta; Efstratiou, Androulla; George, Robert; Stanley, John.

In: Journal of clinical microbiology, Vol. 37, No. 6, 29.05.1999, p. 1948-1952.

Research output: Contribution to journalArticle

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