High resolution mapping of the human sp-a and sp-d loci

R. R. Hoover, Joanna Floros

Research output: Contribution to journalArticle

Abstract

The human pulmonary surfactant protein A (SP-A) locus has been assigned to chromosome 10q22-23 and consists of two nearly identical genes, SP-A1 and SP-A2, as well as a truncated pseudogene. SP-A belongs to the family of C-type lectins along with another surfactant associated protein, SP-D, which has also been mapped to the long arm of chromosome 10. Here we report the relative location of each of the human SP-A and SP-D genomic sequences. Characterization of two overlapping genomic clones revealed that the SP-A pseudogene lies in a reverse orientation 15kb away from the 5' side of SP-A1. This finding was verified by the amplification of the entire SP-A pseudogene / SP-A1 intergenic region using long range PCR with genomic DNA as template. The relative locations of SP-A2 and SP-D were then determined by testing a number of sequence tagged sites (STSs) against the Stanford G3 and TNG3 radiation hybrid panels. The radiation hybrid mapping data showed that both SP-A2 and SP-D are on the 5" side of SP-A1 at approximate distances of 40kb and 120kb, respectively. The SP-A and SP-D loci were also oriented relative to the centromere. The overall order was found to be: centromere-SP-D-SP-A2-pseudogene-SPA1-telomere. Supported by NIH grant HL49823.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997

Fingerprint

Pulmonary Surfactant-Associated Protein D
Pulmonary Surfactant-Associated Protein A
surfactants
Pseudogenes
pseudogenes
varespladib methyl
loci
Radiation Hybrid Mapping
proteins
Centromere
Chromosomes
centromeres
genomics
Pulmonary Surfactant-Associated Proteins
Sequence Tagged Sites
C-Type Lectins
Radiation
Chromosomes, Human, Pair 10
Intergenic DNA
Organized Financing

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

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abstract = "The human pulmonary surfactant protein A (SP-A) locus has been assigned to chromosome 10q22-23 and consists of two nearly identical genes, SP-A1 and SP-A2, as well as a truncated pseudogene. SP-A belongs to the family of C-type lectins along with another surfactant associated protein, SP-D, which has also been mapped to the long arm of chromosome 10. Here we report the relative location of each of the human SP-A and SP-D genomic sequences. Characterization of two overlapping genomic clones revealed that the SP-A pseudogene lies in a reverse orientation 15kb away from the 5' side of SP-A1. This finding was verified by the amplification of the entire SP-A pseudogene / SP-A1 intergenic region using long range PCR with genomic DNA as template. The relative locations of SP-A2 and SP-D were then determined by testing a number of sequence tagged sites (STSs) against the Stanford G3 and TNG3 radiation hybrid panels. The radiation hybrid mapping data showed that both SP-A2 and SP-D are on the 5{"} side of SP-A1 at approximate distances of 40kb and 120kb, respectively. The SP-A and SP-D loci were also oriented relative to the centromere. The overall order was found to be: centromere-SP-D-SP-A2-pseudogene-SPA1-telomere. Supported by NIH grant HL49823.",
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High resolution mapping of the human sp-a and sp-d loci. / Hoover, R. R.; Floros, Joanna.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

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AU - Floros, Joanna

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