High-resolution network biology: Connecting sequence with function

Colm J. Ryan, Peter Cimermančič, Zachary A. Szpiech, Andrej Sali, Ryan D. Hernandez, Nevan J. Krogan

Research output: Contribution to journalReview articlepeer-review

61 Scopus citations

Abstract

Proteins are not monolithic entities; rather, they can contain multiple domains that mediate distinct interactions, and their functionality can be regulated through post-translational modifications at multiple distinct sites. Traditionally, network biology has ignored such properties of proteins and has instead examined either the physical interactions of whole proteins or the consequences of removing entire genes. In this Review, we discuss experimental and computational methods to increase the resolution of protein-protein, genetic and drug-gene interaction studies to the domain and residue levels. Such work will be crucial for using interaction networks to connect sequence and structural information, and to understand the biological consequences of disease-associated mutations, which will hopefully lead to more effective therapeutic strategies.

Original languageEnglish (US)
Pages (from-to)865-879
Number of pages15
JournalNature Reviews Genetics
Volume14
Issue number12
DOIs
StatePublished - Dec 2013

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Fingerprint

Dive into the research topics of 'High-resolution network biology: Connecting sequence with function'. Together they form a unique fingerprint.

Cite this