High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis

Tatiana N. Laremore, Mellisa Ly, Kemal Solakyildirim, Dmitri V. Zagorevski, Robert J. Linhardt

Research output: Contribution to journalArticle

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Abstract

Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6. h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.

Original languageEnglish (US)
Pages (from-to)236-241
Number of pages6
JournalAnalytical Biochemistry
Volume401
Issue number2
DOIs
Publication statusPublished - Jun 1 2010

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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