High-resolution single-molecule kinesin assays at kHz frame rates

Keith J. Mickolajczyk, William O. Hancock

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This chapter describes methods for high-speed, unloaded, in vitro single-molecule kinesin tracking experiments. Instructions are presented for constructing a total internal reflection dark-field microscope (TIRDFM) and labeling motors with gold nanoparticles. An AMP-PNP unlocking assay is introduced as a specialized means of capturing processive events in a reduced field of view. Finally, step-finding tools for analyzing high frame-rate tracking data are described.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-138
Number of pages16
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1805
ISSN (Print)1064-3745

Fingerprint

Adenylyl Imidodiphosphate
Kinesin
Gold
Nanoparticles
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Mickolajczyk, K. J., & Hancock, W. O. (2018). High-resolution single-molecule kinesin assays at kHz frame rates. In Methods in Molecular Biology (pp. 123-138). (Methods in Molecular Biology; Vol. 1805). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8556-2_7
Mickolajczyk, Keith J. ; Hancock, William O. / High-resolution single-molecule kinesin assays at kHz frame rates. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 123-138 (Methods in Molecular Biology).
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Mickolajczyk, KJ & Hancock, WO 2018, High-resolution single-molecule kinesin assays at kHz frame rates. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1805, Humana Press Inc., pp. 123-138. https://doi.org/10.1007/978-1-4939-8556-2_7

High-resolution single-molecule kinesin assays at kHz frame rates. / Mickolajczyk, Keith J.; Hancock, William O.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 123-138 (Methods in Molecular Biology; Vol. 1805).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Mickolajczyk KJ, Hancock WO. High-resolution single-molecule kinesin assays at kHz frame rates. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 123-138. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8556-2_7