High-resolution solid modeling of biological samples imaged with 3D fluorescence microscopy

Michael C. Ferko, Brian W. Patterson, Peter J. Butler

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Optical-sectioning, digital fluorescence microscopy provides images representing temporally- and spatially-resolved molecular-scale details of the substructures of living cells. To render such images into solid models for further computational analyses, we have developed an integrated system of image acquisition, processing, and rendering, which includes a new empirical technique to correct for axial distortions inherent in fluorescence microscopy due to refractive index mismatches between microscope objective immersion medium, coverslip glass, and water. This system takes advantage of the capabilities of ultra-high numerical aperture objectives (e.g. total internal reflection fluorescence microscopy) and enables faithful three-dimensional rendering of living cells into solid models amenable to further computational analysis. An example of solid modeling of bovine aortic endothelial cells and their nuclei is presented. Since many cellular level events are temporally and spatially confined, such integrated image acquisition, processing, rendering, and computational analysis, will enable, in silico, the generation of new computational models for cell mechanics and signaling.

Original languageEnglish (US)
Pages (from-to)648-655
Number of pages8
JournalMicroscopy Research and Technique
Volume69
Issue number8
DOIs
StatePublished - Aug 1 2006

Fingerprint

Fluorescence microscopy
fluorescence microscopy
Fluorescence Microscopy
rendering
Image acquisition
microscopy
fluorescence
high resolution
Cells
acquisition
Refractometry
Endothelial cells
Immersion
Processing
Mechanics
Cell Nucleus
sampling
Computer Simulation
Glass
refractive index

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

Cite this

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High-resolution solid modeling of biological samples imaged with 3D fluorescence microscopy. / Ferko, Michael C.; Patterson, Brian W.; Butler, Peter J.

In: Microscopy Research and Technique, Vol. 69, No. 8, 01.08.2006, p. 648-655.

Research output: Contribution to journalArticle

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