High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells

Xiaonan Yang, Zhuofa Chen, Gihoon Choi, Jun Miao, Liwang Cui, Weihua Guan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This work reports a high throughput and label-free cell deformability microfluidic sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). As a mechanical biomarker, the RBC deformability is highly relevant to the infection status. The cell deformability is measured by evaluating the translocation time when each individual cell squeezes through a microscale constriction. More than 30,000 RBCs can be analyzed for parasitemia quantification in under 1 min with a throughput ∼500 cells/s. Moreover, the device can also differentiate various malaria stages (ring, trophozoite, and schizont stage) due to their varied deformability. As compared to the microscopy and flow cytometry, this microfluidic deformability sensor would allow for label-free and rapid malaria parasitemia quantification and stage determination at a low-cost.

Original languageEnglish (US)
Title of host publication2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages997-1000
Number of pages4
ISBN (Electronic)9781509050789
DOIs
StatePublished - Feb 23 2017
Event30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 - Las Vegas, United States
Duration: Jan 22 2017Jan 26 2017

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
CountryUnited States
CityLas Vegas
Period1/22/171/26/17

Fingerprint

erythrocytes
Formability
Labels
Blood
Cells
Throughput
cells
Microfluidics
cytometry
biomarkers
sensors
infectious diseases
microbalances
Flow cytometry
Sensors
Biomarkers
constrictions
microscopy
Microscopic examination
rings

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Yang, X., Chen, Z., Choi, G., Miao, J., Cui, L., & Guan, W. (2017). High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 (pp. 997-1000). [7863579] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2017.7863579
Yang, Xiaonan ; Chen, Zhuofa ; Choi, Gihoon ; Miao, Jun ; Cui, Liwang ; Guan, Weihua. / High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 997-1000 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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Yang, X, Chen, Z, Choi, G, Miao, J, Cui, L & Guan, W 2017, High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. in 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017., 7863579, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 997-1000, 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017, Las Vegas, United States, 1/22/17. https://doi.org/10.1109/MEMSYS.2017.7863579

High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. / Yang, Xiaonan; Chen, Zhuofa; Choi, Gihoon; Miao, Jun; Cui, Liwang; Guan, Weihua.

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 997-1000 7863579 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Yang X, Chen Z, Choi G, Miao J, Cui L, Guan W. High-throughput and label-free parasitemia quantification and stage determination for plasmodium falciparum-infected red blood cells. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 997-1000. 7863579. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2017.7863579