High-throughput and label-free parasitemia quantification and stage differentiation for malaria-infected red blood cells

Xiaonan Yang, Zhuofa Chen, Jun Miao, Liwang Cui, Weihua Guan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This work reports a high throughput and label-free microfluidic cell deformability sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). The sensor relies on differentiating the RBC deformability (a mechanical biomarker) that is highly correlated with the infection status. The cell deformability is measured by evaluating the transit time when each individual RBC squeezes through a microscale constriction (cross-section ~5 µm×5 µm). More than 30,000 RBCs can be analyzed for parasitemia quantification in under 1 min with a throughput ~500 cells/s. Moreover, the device can also differentiate various malaria stages (ring, trophozoite, and schizont stage) due to their varied deformability. Using Pf-iRBCs at 0.1% parasitemia as a testing sample, the microfluidic deformability sensor achieved an excellent sensitivity (94.29%), specificity (86.67%) and accuracy (92.00%) in a blind test, comparable to the gold standard of the blood smear microscopy. As a supplement technology to the microscopy and flow cytometry, the microfluidic deformability sensor would possibly allow for label-free, rapid and cost-effective parasitemia quantification and stage determination for malaria in remote regions.

Original languageEnglish (US)
Pages (from-to)408-414
Number of pages7
JournalBiosensors and Bioelectronics
Volume98
DOIs
StatePublished - Dec 15 2017

Fingerprint

Parasitemia
Formability
Malaria
Microfluidics
Labels
Blood
Erythrocytes
Cells
Throughput
Plasmodium falciparum
Microscopy
Sensors
Schizonts
Trophozoites
Microscopic examination
Constriction
Flow Cytometry
Flow cytometry
Biomarkers
Technology

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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abstract = "This work reports a high throughput and label-free microfluidic cell deformability sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). The sensor relies on differentiating the RBC deformability (a mechanical biomarker) that is highly correlated with the infection status. The cell deformability is measured by evaluating the transit time when each individual RBC squeezes through a microscale constriction (cross-section ~5 µm×5 µm). More than 30,000 RBCs can be analyzed for parasitemia quantification in under 1 min with a throughput ~500 cells/s. Moreover, the device can also differentiate various malaria stages (ring, trophozoite, and schizont stage) due to their varied deformability. Using Pf-iRBCs at 0.1{\%} parasitemia as a testing sample, the microfluidic deformability sensor achieved an excellent sensitivity (94.29{\%}), specificity (86.67{\%}) and accuracy (92.00{\%}) in a blind test, comparable to the gold standard of the blood smear microscopy. As a supplement technology to the microscopy and flow cytometry, the microfluidic deformability sensor would possibly allow for label-free, rapid and cost-effective parasitemia quantification and stage determination for malaria in remote regions.",
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High-throughput and label-free parasitemia quantification and stage differentiation for malaria-infected red blood cells. / Yang, Xiaonan; Chen, Zhuofa; Miao, Jun; Cui, Liwang; Guan, Weihua.

In: Biosensors and Bioelectronics, Vol. 98, 15.12.2017, p. 408-414.

Research output: Contribution to journalArticle

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