Abstract
Iron has been widely studied in nearly every realm of biology. However, current methodologies, such as genetic mapping or mutation screening, have been difficult to apply due to the lack of robust high-throughput methods for quantifying iron levels from cells or tissues. The measurement of total iron levels in tissues, usually done with atomic absorption spectroscopy, is impractical for large numbers of samples and includes the contribution of heme iron from hemoglobin contained in red blood cells. The measurement of non-heme iron by reaction with a bathophenanthroline reagent, a commonly used assay reported more than 30 years ago, is also not feasible for large-scale analyses because it is cuvette-based. We therefore have modified this method to a microplate format that will facilitate large-scale analysis. The microplate assay is highly sensitive and specific, and is a simple and effective method for the measurement of non-heme iron for animal tissues that will enable the application of high-throughput of genetic methodologies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 195-200 |
| Number of pages | 6 |
| Journal | Journal of Biochemical and Biophysical Methods |
| Volume | 59 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 31 2004 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
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High-throughput non-heme iron assay for animal tissues. / Grundy, Martin A.; Gorman, Nadia; Sinclair, Peter R.; Chorney, Michael J.; Gerhard, Glenn S.
In: Journal of Biochemical and Biophysical Methods, Vol. 59, No. 2, 31.05.2004, p. 195-200.Research output: Contribution to journal › Article
TY - JOUR
T1 - High-throughput non-heme iron assay for animal tissues
AU - Grundy, Martin A.
AU - Gorman, Nadia
AU - Sinclair, Peter R.
AU - Chorney, Michael J.
AU - Gerhard, Glenn S.
PY - 2004/5/31
Y1 - 2004/5/31
N2 - Iron has been widely studied in nearly every realm of biology. However, current methodologies, such as genetic mapping or mutation screening, have been difficult to apply due to the lack of robust high-throughput methods for quantifying iron levels from cells or tissues. The measurement of total iron levels in tissues, usually done with atomic absorption spectroscopy, is impractical for large numbers of samples and includes the contribution of heme iron from hemoglobin contained in red blood cells. The measurement of non-heme iron by reaction with a bathophenanthroline reagent, a commonly used assay reported more than 30 years ago, is also not feasible for large-scale analyses because it is cuvette-based. We therefore have modified this method to a microplate format that will facilitate large-scale analysis. The microplate assay is highly sensitive and specific, and is a simple and effective method for the measurement of non-heme iron for animal tissues that will enable the application of high-throughput of genetic methodologies.
AB - Iron has been widely studied in nearly every realm of biology. However, current methodologies, such as genetic mapping or mutation screening, have been difficult to apply due to the lack of robust high-throughput methods for quantifying iron levels from cells or tissues. The measurement of total iron levels in tissues, usually done with atomic absorption spectroscopy, is impractical for large numbers of samples and includes the contribution of heme iron from hemoglobin contained in red blood cells. The measurement of non-heme iron by reaction with a bathophenanthroline reagent, a commonly used assay reported more than 30 years ago, is also not feasible for large-scale analyses because it is cuvette-based. We therefore have modified this method to a microplate format that will facilitate large-scale analysis. The microplate assay is highly sensitive and specific, and is a simple and effective method for the measurement of non-heme iron for animal tissues that will enable the application of high-throughput of genetic methodologies.
UR - http://www.scopus.com/inward/record.url?scp=2442445100&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442445100&partnerID=8YFLogxK
U2 - 10.1016/j.jbbm.2004.01.001
DO - 10.1016/j.jbbm.2004.01.001
M3 - Article
C2 - 15163531
AN - SCOPUS:2442445100
VL - 59
SP - 195
EP - 200
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 2
ER -