High-throughput non-heme iron assay for animal tissues

Martin A. Grundy, Nadia Gorman, Peter R. Sinclair, Michael J. Chorney, Glenn S. Gerhard

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Iron has been widely studied in nearly every realm of biology. However, current methodologies, such as genetic mapping or mutation screening, have been difficult to apply due to the lack of robust high-throughput methods for quantifying iron levels from cells or tissues. The measurement of total iron levels in tissues, usually done with atomic absorption spectroscopy, is impractical for large numbers of samples and includes the contribution of heme iron from hemoglobin contained in red blood cells. The measurement of non-heme iron by reaction with a bathophenanthroline reagent, a commonly used assay reported more than 30 years ago, is also not feasible for large-scale analyses because it is cuvette-based. We therefore have modified this method to a microplate format that will facilitate large-scale analysis. The microplate assay is highly sensitive and specific, and is a simple and effective method for the measurement of non-heme iron for animal tissues that will enable the application of high-throughput of genetic methodologies.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalJournal of Biochemical and Biophysical Methods
Volume59
Issue number2
DOIs
StatePublished - May 31 2004

Fingerprint

Assays
Animals
Iron
Throughput
Tissue
Atomic spectroscopy
Heme
Absorption spectroscopy
Spectrum Analysis
Screening
Hemoglobins
Blood
Erythrocytes
Cells
Mutation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Cite this

Grundy, M. A., Gorman, N., Sinclair, P. R., Chorney, M. J., & Gerhard, G. S. (2004). High-throughput non-heme iron assay for animal tissues. Journal of Biochemical and Biophysical Methods, 59(2), 195-200. https://doi.org/10.1016/j.jbbm.2004.01.001
Grundy, Martin A. ; Gorman, Nadia ; Sinclair, Peter R. ; Chorney, Michael J. ; Gerhard, Glenn S. / High-throughput non-heme iron assay for animal tissues. In: Journal of Biochemical and Biophysical Methods. 2004 ; Vol. 59, No. 2. pp. 195-200.
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Grundy, MA, Gorman, N, Sinclair, PR, Chorney, MJ & Gerhard, GS 2004, 'High-throughput non-heme iron assay for animal tissues', Journal of Biochemical and Biophysical Methods, vol. 59, no. 2, pp. 195-200. https://doi.org/10.1016/j.jbbm.2004.01.001

High-throughput non-heme iron assay for animal tissues. / Grundy, Martin A.; Gorman, Nadia; Sinclair, Peter R.; Chorney, Michael J.; Gerhard, Glenn S.

In: Journal of Biochemical and Biophysical Methods, Vol. 59, No. 2, 31.05.2004, p. 195-200.

Research output: Contribution to journalArticle

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