High-throughput single molecule detection using nanophotonic/microfluidic hybrid chip

Yanhui Zhao, Danqi Chen, Stephen J. Benkovic, Tony Jun Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ability of nanophotonic hole chips to guide light energy into subwavelength-diameter cylindrical nanoapertures has been exploited for single-molecule fluorescence studies of biomolecules at micromolar concentrations. Here, we present a nanophotonic/microfluidic hybrid chip for single-molecule fluorescence imaging. Our design allowed single-molecule FRET studies that revealed weak DNA-protein and protein-protein interactions found with T4 replisomal proteins.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1811-1813
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - Jan 1 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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  • Cite this

    Zhao, Y., Chen, D., Benkovic, S. J., & Huang, T. J. (2014). High-throughput single molecule detection using nanophotonic/microfluidic hybrid chip. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1811-1813). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.