High variability in quorum quenching and growth inhibition by furanone C-30 in Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients

Rodolfo García-Contreras, Berenice Peréz-Eretza, Ricardo Jasso-Chávez, Elizabeth Lira-Silva, Jesús Alberto Roldán-Sánchez, Abigail González-Valdez, Gloria Sobeŕon-Chávez, Rafael Coria-Jiménez, Mariano Martínez-Vázquez, Luis David Alcaraz, Toshinari Maeda, Thomas Keith Wood

Research output: Contribution to journalArticle

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Abstract

Pseudomonas aeruginosa colonizes the lungs of cystic fibrosis patients causing severe damage. This bacterium is intrinsically resistant to antibiotics and shows resistance against new antimicrobials and its virulence is controlled by the quorum-sensing response. Thus, attenuating its virulence by quorum quenching instead of inhibiting its growth has been proposed to minimize resistance; however, resistance against the canonical quorum quencher furanone C-30 can be achieved by mutations leading to increased efflux. In the present work, the effect of C-30 in the attenuation of the QS-controlled virulence factors elastase and pyocyanin was investigated in 50 isolates from cystic fibrosis patients. The results demonstrate that there is a high variability in the expression of both elastase and pyocyanin and that there are many naturally resistant C-30 strains. We report that the main mechanism of C-30 resistance in these strains was not due to enhanced efflux but a lack of permeability. Moreover, C-30 strongly inhibited the growth of several of the isolates studied, thus imposing high selective pressure for the generation of resistance.

Original languageEnglish (US)
Article numberftv040
JournalPathogens and disease
Volume73
Issue number6
DOIs
StatePublished - Jan 1 2015

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Pyocyanine
Quorum Sensing
Pancreatic Elastase
Cystic Fibrosis
Pseudomonas aeruginosa
Virulence
Virulence Factors
Microbial Drug Resistance
Growth
Permeability
Bacteria
Lung
Mutation
furanone C-30

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

García-Contreras, R., Peréz-Eretza, B., Jasso-Chávez, R., Lira-Silva, E., Roldán-Sánchez, J. A., González-Valdez, A., ... Wood, T. K. (2015). High variability in quorum quenching and growth inhibition by furanone C-30 in Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients. Pathogens and disease, 73(6), [ftv040]. https://doi.org/10.1093/femspd/ftv040
García-Contreras, Rodolfo ; Peréz-Eretza, Berenice ; Jasso-Chávez, Ricardo ; Lira-Silva, Elizabeth ; Roldán-Sánchez, Jesús Alberto ; González-Valdez, Abigail ; Sobeŕon-Chávez, Gloria ; Coria-Jiménez, Rafael ; Martínez-Vázquez, Mariano ; Alcaraz, Luis David ; Maeda, Toshinari ; Wood, Thomas Keith. / High variability in quorum quenching and growth inhibition by furanone C-30 in Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients. In: Pathogens and disease. 2015 ; Vol. 73, No. 6.
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abstract = "Pseudomonas aeruginosa colonizes the lungs of cystic fibrosis patients causing severe damage. This bacterium is intrinsically resistant to antibiotics and shows resistance against new antimicrobials and its virulence is controlled by the quorum-sensing response. Thus, attenuating its virulence by quorum quenching instead of inhibiting its growth has been proposed to minimize resistance; however, resistance against the canonical quorum quencher furanone C-30 can be achieved by mutations leading to increased efflux. In the present work, the effect of C-30 in the attenuation of the QS-controlled virulence factors elastase and pyocyanin was investigated in 50 isolates from cystic fibrosis patients. The results demonstrate that there is a high variability in the expression of both elastase and pyocyanin and that there are many naturally resistant C-30 strains. We report that the main mechanism of C-30 resistance in these strains was not due to enhanced efflux but a lack of permeability. Moreover, C-30 strongly inhibited the growth of several of the isolates studied, thus imposing high selective pressure for the generation of resistance.",
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García-Contreras, R, Peréz-Eretza, B, Jasso-Chávez, R, Lira-Silva, E, Roldán-Sánchez, JA, González-Valdez, A, Sobeŕon-Chávez, G, Coria-Jiménez, R, Martínez-Vázquez, M, Alcaraz, LD, Maeda, T & Wood, TK 2015, 'High variability in quorum quenching and growth inhibition by furanone C-30 in Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients', Pathogens and disease, vol. 73, no. 6, ftv040. https://doi.org/10.1093/femspd/ftv040

High variability in quorum quenching and growth inhibition by furanone C-30 in Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients. / García-Contreras, Rodolfo; Peréz-Eretza, Berenice; Jasso-Chávez, Ricardo; Lira-Silva, Elizabeth; Roldán-Sánchez, Jesús Alberto; González-Valdez, Abigail; Sobeŕon-Chávez, Gloria; Coria-Jiménez, Rafael; Martínez-Vázquez, Mariano; Alcaraz, Luis David; Maeda, Toshinari; Wood, Thomas Keith.

In: Pathogens and disease, Vol. 73, No. 6, ftv040, 01.01.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High variability in quorum quenching and growth inhibition by furanone C-30 in Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients

AU - García-Contreras, Rodolfo

AU - Peréz-Eretza, Berenice

AU - Jasso-Chávez, Ricardo

AU - Lira-Silva, Elizabeth

AU - Roldán-Sánchez, Jesús Alberto

AU - González-Valdez, Abigail

AU - Sobeŕon-Chávez, Gloria

AU - Coria-Jiménez, Rafael

AU - Martínez-Vázquez, Mariano

AU - Alcaraz, Luis David

AU - Maeda, Toshinari

AU - Wood, Thomas Keith

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Pseudomonas aeruginosa colonizes the lungs of cystic fibrosis patients causing severe damage. This bacterium is intrinsically resistant to antibiotics and shows resistance against new antimicrobials and its virulence is controlled by the quorum-sensing response. Thus, attenuating its virulence by quorum quenching instead of inhibiting its growth has been proposed to minimize resistance; however, resistance against the canonical quorum quencher furanone C-30 can be achieved by mutations leading to increased efflux. In the present work, the effect of C-30 in the attenuation of the QS-controlled virulence factors elastase and pyocyanin was investigated in 50 isolates from cystic fibrosis patients. The results demonstrate that there is a high variability in the expression of both elastase and pyocyanin and that there are many naturally resistant C-30 strains. We report that the main mechanism of C-30 resistance in these strains was not due to enhanced efflux but a lack of permeability. Moreover, C-30 strongly inhibited the growth of several of the isolates studied, thus imposing high selective pressure for the generation of resistance.

AB - Pseudomonas aeruginosa colonizes the lungs of cystic fibrosis patients causing severe damage. This bacterium is intrinsically resistant to antibiotics and shows resistance against new antimicrobials and its virulence is controlled by the quorum-sensing response. Thus, attenuating its virulence by quorum quenching instead of inhibiting its growth has been proposed to minimize resistance; however, resistance against the canonical quorum quencher furanone C-30 can be achieved by mutations leading to increased efflux. In the present work, the effect of C-30 in the attenuation of the QS-controlled virulence factors elastase and pyocyanin was investigated in 50 isolates from cystic fibrosis patients. The results demonstrate that there is a high variability in the expression of both elastase and pyocyanin and that there are many naturally resistant C-30 strains. We report that the main mechanism of C-30 resistance in these strains was not due to enhanced efflux but a lack of permeability. Moreover, C-30 strongly inhibited the growth of several of the isolates studied, thus imposing high selective pressure for the generation of resistance.

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García-Contreras R, Peréz-Eretza B, Jasso-Chávez R, Lira-Silva E, Roldán-Sánchez JA, González-Valdez A et al. High variability in quorum quenching and growth inhibition by furanone C-30 in Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients. Pathogens and disease. 2015 Jan 1;73(6). ftv040. https://doi.org/10.1093/femspd/ftv040