High yield expression and secretion of the ovine pregnancy recognition hormone interferon-τ by Pichia pastoris

Gino Van Heeke, Troy L. Ott, Andre Strauss, Domenico Ammaturo, Fuller W. Bazer

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

The early conceptus (embryo and associated membranes) of domestic ruminants signals its presence to the maternal uterus through production of interferon-τ (IFN-τ). Production of IFN-τ ensures continued production of progesterone, the hormone of pregnancy, by the ovarian corpus luteum. This paper reports the high-level expression and efficient secretion of biologically active recombinant ovine IFN-τ (rOvIFN-τ) by Pichia pastoris. The developed method produces more than 80% pure recombinant ovine IFN-τ, obviating the need for further purification for many purposes. Initial fermentation studies produced IFN-τ at 280 mg/liter and demonstrate the potential of this system for large-scale production of IFN-τ.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalJournal of Interferon and Cytokine Research
Volume16
Issue number2
DOIs
StatePublished - Feb 1996

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology

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