@article{10c0d8752908418b973863f99c703fdb,
title = "High-yield production of extracellular type-I cellulose by the cyanobacterium Synechococcus sp. PCC 7002",
abstract = "Cellulose synthase, encoded by the cesA gene, is responsible for the synthesis of cellulose in nature. We show that the cell wall of the cyanobacterium Synechococcus sp. PCC 7002 naturally contains cellulose. Cellulose occurs as a possibly laminated layer between the inner and outer membrane, as well as being an important component of the extracellular glycocalyx in this cyanobacterium. Overexpression of six genes, cmc-ccp-cesAB-cesC-cesD-bgl, from Gluconacetobacter xylinus in Synechococcus sp. PCC 7002 resulted in very high-yield production of extracellular type-I cellulose. High-level cellulose production only occurred when the native cesA gene was inactivated and when cells were grown at low salinity. This system provides a method for the production of lignin-free cellulose from sunlight and CO2 for biofuel production and other biotechnological applications.",
author = "Chi Zhao and Zhongkui Li and Tao Li and Yingjiao Zhang and Bryant, {Donald A.} and Jindong Zhao",
note = "Funding Information: This work was supported by grants from the Ministry of Science and Technology of China (2009AA021405 and 2015CD150101) and the CAS International Partnership Program for Research Teams to JZ. Work in the laboratory of DAB was supported by Air Force Office of Scientific Research MURI grant FA9550-05-1-0365. We thank Nicole Zembower and Susan Magargee for assistance with confocal imaging, and Dr. Gang Ning and Missy Hazen at the Microscopy and Cytometry Facility of the Huck Institutes of the Life Sciences (Penn State University, University Park), Yingchun Hu at College of Life Sciences, Peking University and Jingnan Liang at Institute of Microbiology, Chinese Academy of Sciences for the help with transmission electron microscopy, Chunli Li at Institute of Microbiology, Chinese Academy of Sciences for the help with scanning electron microscopy, and Fuhui Liao at College of Chemistry & Molecular Engineering, Peking University for help with X-ray diffraction. Publisher Copyright: {\textcopyright} 2015 SIBS, CAS All rights reserved.",
year = "2015",
month = apr,
day = "28",
doi = "10.1038/celldisc.2015.4",
language = "English (US)",
volume = "1",
journal = "Cell Discovery",
issn = "2056-5968",
publisher = "Nature Publishing Group",
}