Highly specific and sensitive non-enzymatic determination of uric acid in serum and urine by extended gate field effect transistor sensors

Weihua Guan, Xuexin Duan, Mark A. Reed

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

A potentiometric non-enzymatic sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode is demonstrated for determining the uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. This potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific. The interference that comes from glucose, bilirubin, ascorbic acid and hemoglobin is negligible in normal concentration range of these interferents. The sensor also exhibits excellent long term reliability. This extended gate field effect transistor based sensors can be used as a point of care UA testing tool, due to the small size, low cost, and low sample volume consumption.

Original languageEnglish (US)
Pages (from-to)225-231
Number of pages7
JournalBiosensors and Bioelectronics
Volume51
DOIs
StatePublished - Jan 5 2014

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Fingerprint Dive into the research topics of 'Highly specific and sensitive non-enzymatic determination of uric acid in serum and urine by extended gate field effect transistor sensors'. Together they form a unique fingerprint.

Cite this