TY - JOUR
T1 - Highly specific and sensitive non-enzymatic determination of uric acid in serum and urine by extended gate field effect transistor sensors
AU - Guan, Weihua
AU - Duan, Xuexin
AU - Reed, Mark A.
N1 - Funding Information:
We would like to thank Michael Power for the help during the device fabrication process and Amar Agashe and Nathan Long for the long term reliability measurement. W.G. acknowledges the support from the Howard Hughes Medical Institute International Student Research Fellowship . The work was supported in part by the Defense Threat Reduction Agency under Grants HDTRA1-10-1-0037 and HDTRA-1-12-1-0042 , and by the U.S. Army Research Laboratory and the U.S. Army Research Office under Contract/Grant number MURI W911NF-11-1-0024 . Facilities used were supported by Yale Institute for Nanoscience and Quantum Engineering and NSF MRSEC DMR 1119826 .
PY - 2014/1/5
Y1 - 2014/1/5
N2 - A potentiometric non-enzymatic sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode is demonstrated for determining the uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. This potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific. The interference that comes from glucose, bilirubin, ascorbic acid and hemoglobin is negligible in normal concentration range of these interferents. The sensor also exhibits excellent long term reliability. This extended gate field effect transistor based sensors can be used as a point of care UA testing tool, due to the small size, low cost, and low sample volume consumption.
AB - A potentiometric non-enzymatic sensor using off-chip extended-gate field effect transistor (EGFET) with a ferrocenyl-alkanethiol modified gold electrode is demonstrated for determining the uric acid concentration in human serum and urine. Hexacyanoferrate (II) and (III) ions are used as redox reagent. This potentiometric sensor measures the interface potential on the ferrocene immobilized gold electrode, which is modulated by the redox reaction between uric acid and hexacyanoferrate ions. The device shows a near Nernstian response to uric acid and is highly specific. The interference that comes from glucose, bilirubin, ascorbic acid and hemoglobin is negligible in normal concentration range of these interferents. The sensor also exhibits excellent long term reliability. This extended gate field effect transistor based sensors can be used as a point of care UA testing tool, due to the small size, low cost, and low sample volume consumption.
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U2 - 10.1016/j.bios.2013.07.061
DO - 10.1016/j.bios.2013.07.061
M3 - Article
C2 - 23968728
AN - SCOPUS:84882765005
VL - 51
SP - 225
EP - 231
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -