Highly stable enzyme precipitate coatings and their electrochemical applications

Byoung Chan Kim, Xueyan Zhao, Hye Kyung Ahn, Jae Hyun Kim, Hye Jin Lee, Kyung Woo Kim, Sujith Nair, Erik Hsiao, Hongfei Jia, Min Kyu Oh, Byoung In Sang, Beom Soo Kim, Seong H. Kim, Yongchai Kwon, Su Ha, Man Bock Gu, Ping Wang, Jungbae Kim

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

This paper describes highly stable enzyme precipitate coatings (EPCs) on electrospun polymer nanofibers and carbon nanotubes (CNTs), and their potential applications in the development of highly sensitive biosensors and high-powered biofuel cells. EPCs of glucose oxidase (GOx) were prepared by precipitating GOx molecules in the presence of ammonium sulfate, then cross-linking the precipitated GOx aggregates on covalently attached enzyme molecules on the surface of nanomaterials. EPCs-GOx not only improved enzyme loading, but also retained high enzyme stability. For example, EPC-GOx on CNTs showed a 50 times higher activity per unit weight of CNTs than the conventional approach of covalent attachment, and its initial activity was maintained with negligible loss for 200 days. EPC-GOx on CNTs was entrapped by Nafion to prepare enzyme electrodes for glucose sensors and biofuel cells. The EPC-GOx electrode showed a higher sensitivity and a lower detection limit than an electrode prepared with covalently attached GOx (CA-GOx). The CA-GOx electrode showed an 80% drop in sensitivity after thermal treatment at 50°C for 4. h, while the EPC-GOx electrode maintained its high sensitivity with negligible decrease under the same conditions. The use of EPC-GOx as the anode of a biofuel cell improved the power density, which was also stable even after thermal treatment of the enzyme anode at 50°C. The excellent stability of the EPC-GOx electrode together with its high current output create new potential for the practical applications of enzyme-based glucose sensors and biofuel cells.

Original languageEnglish (US)
Pages (from-to)1980-1986
Number of pages7
JournalBiosensors and Bioelectronics
Volume26
Issue number5
DOIs
StatePublished - Jan 15 2011

Fingerprint

Glucose Oxidase
Glucose oxidase
Precipitates
Enzymes
Coatings
Bioelectric Energy Sources
Electrodes
Biological fuel cells
Carbon Nanotubes
Carbon nanotubes
Enzyme Stability
Glucose sensors
Biosensing Techniques
Hot Temperature
Anodes
Nanofibers
Glucose
Heat treatment
Enzyme electrodes
Nanostructures

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Kim, B. C., Zhao, X., Ahn, H. K., Kim, J. H., Lee, H. J., Kim, K. W., ... Kim, J. (2011). Highly stable enzyme precipitate coatings and their electrochemical applications. Biosensors and Bioelectronics, 26(5), 1980-1986. https://doi.org/10.1016/j.bios.2010.08.068
Kim, Byoung Chan ; Zhao, Xueyan ; Ahn, Hye Kyung ; Kim, Jae Hyun ; Lee, Hye Jin ; Kim, Kyung Woo ; Nair, Sujith ; Hsiao, Erik ; Jia, Hongfei ; Oh, Min Kyu ; Sang, Byoung In ; Kim, Beom Soo ; Kim, Seong H. ; Kwon, Yongchai ; Ha, Su ; Gu, Man Bock ; Wang, Ping ; Kim, Jungbae. / Highly stable enzyme precipitate coatings and their electrochemical applications. In: Biosensors and Bioelectronics. 2011 ; Vol. 26, No. 5. pp. 1980-1986.
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abstract = "This paper describes highly stable enzyme precipitate coatings (EPCs) on electrospun polymer nanofibers and carbon nanotubes (CNTs), and their potential applications in the development of highly sensitive biosensors and high-powered biofuel cells. EPCs of glucose oxidase (GOx) were prepared by precipitating GOx molecules in the presence of ammonium sulfate, then cross-linking the precipitated GOx aggregates on covalently attached enzyme molecules on the surface of nanomaterials. EPCs-GOx not only improved enzyme loading, but also retained high enzyme stability. For example, EPC-GOx on CNTs showed a 50 times higher activity per unit weight of CNTs than the conventional approach of covalent attachment, and its initial activity was maintained with negligible loss for 200 days. EPC-GOx on CNTs was entrapped by Nafion to prepare enzyme electrodes for glucose sensors and biofuel cells. The EPC-GOx electrode showed a higher sensitivity and a lower detection limit than an electrode prepared with covalently attached GOx (CA-GOx). The CA-GOx electrode showed an 80{\%} drop in sensitivity after thermal treatment at 50°C for 4. h, while the EPC-GOx electrode maintained its high sensitivity with negligible decrease under the same conditions. The use of EPC-GOx as the anode of a biofuel cell improved the power density, which was also stable even after thermal treatment of the enzyme anode at 50°C. The excellent stability of the EPC-GOx electrode together with its high current output create new potential for the practical applications of enzyme-based glucose sensors and biofuel cells.",
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Kim, BC, Zhao, X, Ahn, HK, Kim, JH, Lee, HJ, Kim, KW, Nair, S, Hsiao, E, Jia, H, Oh, MK, Sang, BI, Kim, BS, Kim, SH, Kwon, Y, Ha, S, Gu, MB, Wang, P & Kim, J 2011, 'Highly stable enzyme precipitate coatings and their electrochemical applications', Biosensors and Bioelectronics, vol. 26, no. 5, pp. 1980-1986. https://doi.org/10.1016/j.bios.2010.08.068

Highly stable enzyme precipitate coatings and their electrochemical applications. / Kim, Byoung Chan; Zhao, Xueyan; Ahn, Hye Kyung; Kim, Jae Hyun; Lee, Hye Jin; Kim, Kyung Woo; Nair, Sujith; Hsiao, Erik; Jia, Hongfei; Oh, Min Kyu; Sang, Byoung In; Kim, Beom Soo; Kim, Seong H.; Kwon, Yongchai; Ha, Su; Gu, Man Bock; Wang, Ping; Kim, Jungbae.

In: Biosensors and Bioelectronics, Vol. 26, No. 5, 15.01.2011, p. 1980-1986.

Research output: Contribution to journalArticle

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T1 - Highly stable enzyme precipitate coatings and their electrochemical applications

AU - Kim, Byoung Chan

AU - Zhao, Xueyan

AU - Ahn, Hye Kyung

AU - Kim, Jae Hyun

AU - Lee, Hye Jin

AU - Kim, Kyung Woo

AU - Nair, Sujith

AU - Hsiao, Erik

AU - Jia, Hongfei

AU - Oh, Min Kyu

AU - Sang, Byoung In

AU - Kim, Beom Soo

AU - Kim, Seong H.

AU - Kwon, Yongchai

AU - Ha, Su

AU - Gu, Man Bock

AU - Wang, Ping

AU - Kim, Jungbae

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N2 - This paper describes highly stable enzyme precipitate coatings (EPCs) on electrospun polymer nanofibers and carbon nanotubes (CNTs), and their potential applications in the development of highly sensitive biosensors and high-powered biofuel cells. EPCs of glucose oxidase (GOx) were prepared by precipitating GOx molecules in the presence of ammonium sulfate, then cross-linking the precipitated GOx aggregates on covalently attached enzyme molecules on the surface of nanomaterials. EPCs-GOx not only improved enzyme loading, but also retained high enzyme stability. For example, EPC-GOx on CNTs showed a 50 times higher activity per unit weight of CNTs than the conventional approach of covalent attachment, and its initial activity was maintained with negligible loss for 200 days. EPC-GOx on CNTs was entrapped by Nafion to prepare enzyme electrodes for glucose sensors and biofuel cells. The EPC-GOx electrode showed a higher sensitivity and a lower detection limit than an electrode prepared with covalently attached GOx (CA-GOx). The CA-GOx electrode showed an 80% drop in sensitivity after thermal treatment at 50°C for 4. h, while the EPC-GOx electrode maintained its high sensitivity with negligible decrease under the same conditions. The use of EPC-GOx as the anode of a biofuel cell improved the power density, which was also stable even after thermal treatment of the enzyme anode at 50°C. The excellent stability of the EPC-GOx electrode together with its high current output create new potential for the practical applications of enzyme-based glucose sensors and biofuel cells.

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