Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations

G. T. Slavov, G. T. Howe, I. Yakovlev, K. J. Edwards, K. V. Krutovskii, G. A. Tuskan, J. E. Carlson, S. H. Strauss, W. T. Adams

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Twenty-two highly variable SSR markers were developed in Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] from five SSR-enriched genomic libraries. Fifteen PCR primer pairs amplified a single codominant locus, while seven primer pairs occasionally amplified two loci. The Mendelian inheritance of all 22 SSRs was confirmed via segregation analyses in several Douglas-fir families. The mean observed heterozygosity and the mean number of alleles per locus were 0.855 (SE=0.020) and 23 (SE=1.6), respectively. Twenty markers were used in genetic linkage analysis and mapped to ten known linkage groups. Because of their high polymorphism and unambiguous phenotypes, 15 single-locus markers were selected as the most suitable for DNA fingerprinting and parentage analysis. Only three SSRs were sufficient to achieve an average probability of exclusion from paternity of 0.998 in a Douglas-fir seed orchard block consisting of 59 parents.

Original languageEnglish (US)
Pages (from-to)873-880
Number of pages8
JournalTheoretical and Applied Genetics
Volume108
Issue number5
DOIs
StatePublished - Mar 2004

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Fingerprint Dive into the research topics of 'Highly variable SSR markers in Douglas-fir: Mendelian inheritance and map locations'. Together they form a unique fingerprint.

Cite this