Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells

Thomas W. Gardner, Treena Lesher, Sonny Khin, Cuong Vu, Alistair J. Barber, William A. Brennan

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10-9-10-4 M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H1 and H2 receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.

Original languageEnglish (US)
Pages (from-to)717-721
Number of pages5
JournalBiochemical Journal
Volume320
Issue number3
DOIs
StatePublished - Dec 15 1996

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Zonula Occludens-1 Protein
Endothelial cells
Histamine
Tight Junction Proteins
Endothelial Cells
Histamine H1 Receptors
Retinal Vessels
Histamine H2 Receptors
Capillary Permeability
Permeability
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Gardner, Thomas W. ; Lesher, Treena ; Khin, Sonny ; Vu, Cuong ; Barber, Alistair J. ; Brennan, William A. / Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells. In: Biochemical Journal. 1996 ; Vol. 320, No. 3. pp. 717-721.
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Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells. / Gardner, Thomas W.; Lesher, Treena; Khin, Sonny; Vu, Cuong; Barber, Alistair J.; Brennan, William A.

In: Biochemical Journal, Vol. 320, No. 3, 15.12.1996, p. 717-721.

Research output: Contribution to journalArticle

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