Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells

Thomas W. Gardner, Treena Lesher, Sonny Khin, Cuong Vu, Alistair J. Barber, William A. Brennan

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10-9-10-4 M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H1 and H2 receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.

Original languageEnglish (US)
Pages (from-to)717-721
Number of pages5
JournalBiochemical Journal
Volume320
Issue number3
DOIs
StatePublished - Dec 15 1996

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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