Histidine regulation in Salmonella typhimurium: an activator attenuator model of gene regulation

S. W. Artz, James Broach

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

An activator attenuator model of positive control, as opposed to the classic repressor operator model of negative control, is proposed for the major operon specific mechanism governing expression of the histidine gene cluster of S. typhimurium. Evidence for this mechanism is derived from experiments performed with a coupled in vitro transcription translation system, as well as with a minimal in vitro transcription system. The product (G enzyme, or N 1 [5' phosphoribosyl] adenosine triphosphate:pyrophosphate phosphoribosyl transferase; EC 2.4.2.17) of the first structural gene (hisG) of the histidine operon is not involved in the positive control mechanism. However, a possible role for G enzyme as an accessory negative control element interacting at the attenuator can be accommodated in our model. The operon specific mechanism works in conjunction with an independent mechanism involving guanosine 5' diphosphate 3' diphosphate (ppGpp) which appears to be a positive effector involved in regulating amino acid producing systems in general.

Original languageEnglish (US)
Pages (from-to)3453-3457
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number9
DOIs
StatePublished - Jan 1 1975

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Salmonella typhimurium
Operon
Histidine
ATP Phosphoribosyltransferase
Guanosine Tetraphosphate
Genes
Enzymes
Multigene Family
Transferases
Adenosine Diphosphate
Adenosine Triphosphate
Amino Acids
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • General

Cite this

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