Histiotypic organization and cell differentiation in rat retinal reaggregate cultures

Kimio Akagawa, David Hicks, Colin Barnstable

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Reaggregate cultures have been formed from cell suspensions of enonatal rat retinas. Histological sections of the reaggregates showed evidence of lamination with central rosettes formed around a lumen, a clear neuropil layer and an outer cellular layer. Each of the major retinal cell types, except ganglion cells, could be positively identified using cell type-specific antibodies to label cryostat sections. Many of these were found to occupy positions within the reaggregates similar to those found in the intact retina. Electron microscopic observations showed abundant immature and mature synaptic endings within the neuropil layer, including a number of ribbon synapses. Examination of the rosettes showed an arrangement of Müller glia and photoreceptors that closely resembled that of the intact retina. Within the lumen of rosettes, photoreceptors were found to contain stacks of disc-like membranes bounded by a plasma membrane, analogous to immature outer segments. The photoreceptors within rosettes also underwent molecular differentiation and expressed an outer segment specific marker. The findings suggest that retinal cells have intrinsic properties that allow them to organize themselves into a correctly laminated structure and that particular cell interactions are necessary for continued differentiation of at least rod photoreceptors and Müller cells.

Original languageEnglish (US)
Pages (from-to)298-308
Number of pages11
JournalBrain research
Volume437
Issue number2
DOIs
StatePublished - Dec 29 1987

Fingerprint

Cell Differentiation
Retina
Neuropil
Retinal Rod Photoreceptor Cells
Retinal Ganglion Cells
Cell Communication
Neuroglia
Synapses
Suspensions
Cell Membrane
Electrons
Membranes
Antibodies

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

Cite this

@article{cd070b4b8101473499143859f43e2876,
title = "Histiotypic organization and cell differentiation in rat retinal reaggregate cultures",
abstract = "Reaggregate cultures have been formed from cell suspensions of enonatal rat retinas. Histological sections of the reaggregates showed evidence of lamination with central rosettes formed around a lumen, a clear neuropil layer and an outer cellular layer. Each of the major retinal cell types, except ganglion cells, could be positively identified using cell type-specific antibodies to label cryostat sections. Many of these were found to occupy positions within the reaggregates similar to those found in the intact retina. Electron microscopic observations showed abundant immature and mature synaptic endings within the neuropil layer, including a number of ribbon synapses. Examination of the rosettes showed an arrangement of M{\"u}ller glia and photoreceptors that closely resembled that of the intact retina. Within the lumen of rosettes, photoreceptors were found to contain stacks of disc-like membranes bounded by a plasma membrane, analogous to immature outer segments. The photoreceptors within rosettes also underwent molecular differentiation and expressed an outer segment specific marker. The findings suggest that retinal cells have intrinsic properties that allow them to organize themselves into a correctly laminated structure and that particular cell interactions are necessary for continued differentiation of at least rod photoreceptors and M{\"u}ller cells.",
author = "Kimio Akagawa and David Hicks and Colin Barnstable",
year = "1987",
month = "12",
day = "29",
doi = "10.1016/0006-8993(87)91644-1",
language = "English (US)",
volume = "437",
pages = "298--308",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

Histiotypic organization and cell differentiation in rat retinal reaggregate cultures. / Akagawa, Kimio; Hicks, David; Barnstable, Colin.

In: Brain research, Vol. 437, No. 2, 29.12.1987, p. 298-308.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Histiotypic organization and cell differentiation in rat retinal reaggregate cultures

AU - Akagawa, Kimio

AU - Hicks, David

AU - Barnstable, Colin

PY - 1987/12/29

Y1 - 1987/12/29

N2 - Reaggregate cultures have been formed from cell suspensions of enonatal rat retinas. Histological sections of the reaggregates showed evidence of lamination with central rosettes formed around a lumen, a clear neuropil layer and an outer cellular layer. Each of the major retinal cell types, except ganglion cells, could be positively identified using cell type-specific antibodies to label cryostat sections. Many of these were found to occupy positions within the reaggregates similar to those found in the intact retina. Electron microscopic observations showed abundant immature and mature synaptic endings within the neuropil layer, including a number of ribbon synapses. Examination of the rosettes showed an arrangement of Müller glia and photoreceptors that closely resembled that of the intact retina. Within the lumen of rosettes, photoreceptors were found to contain stacks of disc-like membranes bounded by a plasma membrane, analogous to immature outer segments. The photoreceptors within rosettes also underwent molecular differentiation and expressed an outer segment specific marker. The findings suggest that retinal cells have intrinsic properties that allow them to organize themselves into a correctly laminated structure and that particular cell interactions are necessary for continued differentiation of at least rod photoreceptors and Müller cells.

AB - Reaggregate cultures have been formed from cell suspensions of enonatal rat retinas. Histological sections of the reaggregates showed evidence of lamination with central rosettes formed around a lumen, a clear neuropil layer and an outer cellular layer. Each of the major retinal cell types, except ganglion cells, could be positively identified using cell type-specific antibodies to label cryostat sections. Many of these were found to occupy positions within the reaggregates similar to those found in the intact retina. Electron microscopic observations showed abundant immature and mature synaptic endings within the neuropil layer, including a number of ribbon synapses. Examination of the rosettes showed an arrangement of Müller glia and photoreceptors that closely resembled that of the intact retina. Within the lumen of rosettes, photoreceptors were found to contain stacks of disc-like membranes bounded by a plasma membrane, analogous to immature outer segments. The photoreceptors within rosettes also underwent molecular differentiation and expressed an outer segment specific marker. The findings suggest that retinal cells have intrinsic properties that allow them to organize themselves into a correctly laminated structure and that particular cell interactions are necessary for continued differentiation of at least rod photoreceptors and Müller cells.

UR - http://www.scopus.com/inward/record.url?scp=0023598124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023598124&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(87)91644-1

DO - 10.1016/0006-8993(87)91644-1

M3 - Article

C2 - 3435839

AN - SCOPUS:0023598124

VL - 437

SP - 298

EP - 308

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -