Histological-MRI correlation in the primary motor cortex of patients with amyotrophic lateral sclerosis

Mark Meadowcroft, Nathan J. Mutic, Don C. Bigler, Jian-li Wang, Zachary Simmons, James Connor, Qing Yang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: To establish the relationship between ALS histopathology and quantitative MRI metrics. Materials and Methods: ALS patients (N = 8) in advanced stages of the disease were enrolled and, immediately after death, the brain of each patient was removed. Freshly excised ALS tissue was imaged at 3.0 Tesla with T1and T2 mapping protocols and subsequently stained with astrocyte, myelin, and neuronal markers. Measures of ALS histological stains were compared with the internal control (primary visual cortex) and longitudinal parametric maps. Results: Post-mortem T1-weighted images demonstrate diminished contrast between gray and white matter and alterations in T1 relaxation within the primary motor cortex. An increase in astrocyte number and reactivity as well as evident neuronal loss, a decrease in axonal density, and unraveling of the myelin sheaths in subcortical white matter were found in the ALS primary motor cortex exhibiting significant T1 relaxation and contrast changes. Conclusion: This study provides a histopathological basis for differences in MR T1 contrast and relaxation seen in the ALS brain.

Original languageEnglish (US)
Pages (from-to)665-675
Number of pages11
JournalJournal of Magnetic Resonance Imaging
Volume41
Issue number3
DOIs
StatePublished - Mar 1 2015

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Amyotrophic Lateral Sclerosis
Motor Cortex
Myelin Sheath
Astrocytes
Brain Death
Visual Cortex
Coloring Agents
Brain
White Matter
Gray Matter

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

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abstract = "Purpose: To establish the relationship between ALS histopathology and quantitative MRI metrics. Materials and Methods: ALS patients (N = 8) in advanced stages of the disease were enrolled and, immediately after death, the brain of each patient was removed. Freshly excised ALS tissue was imaged at 3.0 Tesla with T1and T2 mapping protocols and subsequently stained with astrocyte, myelin, and neuronal markers. Measures of ALS histological stains were compared with the internal control (primary visual cortex) and longitudinal parametric maps. Results: Post-mortem T1-weighted images demonstrate diminished contrast between gray and white matter and alterations in T1 relaxation within the primary motor cortex. An increase in astrocyte number and reactivity as well as evident neuronal loss, a decrease in axonal density, and unraveling of the myelin sheaths in subcortical white matter were found in the ALS primary motor cortex exhibiting significant T1 relaxation and contrast changes. Conclusion: This study provides a histopathological basis for differences in MR T1 contrast and relaxation seen in the ALS brain.",
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Histological-MRI correlation in the primary motor cortex of patients with amyotrophic lateral sclerosis. / Meadowcroft, Mark; Mutic, Nathan J.; Bigler, Don C.; Wang, Jian-li; Simmons, Zachary; Connor, James; Yang, Qing.

In: Journal of Magnetic Resonance Imaging, Vol. 41, No. 3, 01.03.2015, p. 665-675.

Research output: Contribution to journalArticle

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