Histone deacetylase 6 regulates cytokinesis and erythrocyte enucleation through deacetylation of formin protein mDia2

Xuehui Li, Yang Mei, Bowen Yan, Eric Vitriol, Suming Huang, Peng Ji, Yi Qiu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The formin protein mDia2 plays a critical role in a number of cellular processes through its ability to promote nucleation and elongation of actin filaments. In erythroblasts, this includes control of cytokinesis and enucleation by regulating contractile actin ring formation. Here we report a novel mechanism of how mDia2 is regulated: through acetylation and deacetylation at lysine 970 in the formin homology 2 domain. Ectopic expression of an acetyl-mimic mDia2 mutant in mouse erythroblasts is sufficient to abolish contractile actin ring formation at the cleavage furrow and subsequent erythrocyte cytokinesis and enucleation. We also identified that class II histone deacetylase 6 deacetylates and subsequently activates mDia2. Knockdown or inhibition of histone deacetylase 6 impairs contractile actin ring formation, and expression of a non-acetyl-mimic mDia2 mutant restores the contractile actin ring and rescues the impairment of enucleation. In addition to revealing a new step in mDia2 regulation, this study may unveil a novel regulatory mechanism of formin-mediated actin assembly, since the K970 acetylation site is conserved among Dia proteins

Original languageEnglish (US)
Pages (from-to)984-994
Number of pages11
JournalHaematologica
Volume102
Issue number6
DOIs
StatePublished - Jan 1 2017

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Histone Deacetylases
Cytokinesis
Actins
Erythrocytes
Erythroblasts
Acetylation
Proteins
Actin Cytoskeleton
Lysine

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Li, Xuehui ; Mei, Yang ; Yan, Bowen ; Vitriol, Eric ; Huang, Suming ; Ji, Peng ; Qiu, Yi. / Histone deacetylase 6 regulates cytokinesis and erythrocyte enucleation through deacetylation of formin protein mDia2. In: Haematologica. 2017 ; Vol. 102, No. 6. pp. 984-994.
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Histone deacetylase 6 regulates cytokinesis and erythrocyte enucleation through deacetylation of formin protein mDia2. / Li, Xuehui; Mei, Yang; Yan, Bowen; Vitriol, Eric; Huang, Suming; Ji, Peng; Qiu, Yi.

In: Haematologica, Vol. 102, No. 6, 01.01.2017, p. 984-994.

Research output: Contribution to journalArticle

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