Histone neighbors in nuclei and extended chromatin

Ross Cameron Hardison, Deborah Parris Zeitler, Joyce M. Murphy, Roger Chalkley

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Histone neighbors in compact and extended chromatin have been investigated by cross-linking histones in nuclei and in nucleohistone extended with 6 M urea, using the bifunctional reversible reagent methyl-4-mercaptobutyrimidate (MMB). Similar histone dimers are found in both conformational states of chromatin. The dimers most frequently found are H2b-H2a, H2b-H3 and H3-H2a; dimers found less frequently are H3-H4, H3-H3 and H2b-H4. More H3-H3 is found in nuclei than in extended chromatin. H1 is found predominantly as poly-H1, although it can be cross-linked to H2b or H3. After reaction with MMB, native compact chromatin is no longer extendable in 6 M urea, which shows that the reagent is capable of linking together histones holding the chromatin in a compact conformation. Thus the histone propinquity in extended chromatin mimics the intimate histone associations in compact chromatin.

Original languageEnglish (US)
Pages (from-to)417-427
Number of pages11
JournalCell
Volume12
Issue number2
DOIs
StatePublished - Jan 1 1977

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Histones
Chromatin
Dimers
Urea
Cross-Linking Reagents
Conformations

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hardison, R. C., Zeitler, D. P., Murphy, J. M., & Chalkley, R. (1977). Histone neighbors in nuclei and extended chromatin. Cell, 12(2), 417-427. https://doi.org/10.1016/0092-8674(77)90118-0
Hardison, Ross Cameron ; Zeitler, Deborah Parris ; Murphy, Joyce M. ; Chalkley, Roger. / Histone neighbors in nuclei and extended chromatin. In: Cell. 1977 ; Vol. 12, No. 2. pp. 417-427.
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Hardison, RC, Zeitler, DP, Murphy, JM & Chalkley, R 1977, 'Histone neighbors in nuclei and extended chromatin', Cell, vol. 12, no. 2, pp. 417-427. https://doi.org/10.1016/0092-8674(77)90118-0

Histone neighbors in nuclei and extended chromatin. / Hardison, Ross Cameron; Zeitler, Deborah Parris; Murphy, Joyce M.; Chalkley, Roger.

In: Cell, Vol. 12, No. 2, 01.01.1977, p. 417-427.

Research output: Contribution to journalArticle

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