HIV-1 and two avian retroviral 5' untranslated regions bind orthologous human and chicken RNA binding proteins

Matthew Stake, Deepali Singh, Gatikrushna Singh, J. Marcela Hernandez, Rebecca Kaddis Maldonado, Leslie J. Parent, Kathleen Boris-Lawrie

Research output: Contribution to journalArticle

  • 3 Citations

Abstract

Essential host cofactors in retrovirus replication bind cis-acting sequences in the 5'untranslated region (UTR). Although host RBPs are crucial to all aspects of virus biology, elucidating their roles in replication remains a challenge to the field. Here RNA affinity-coupled-proteomics generated a comprehensive, unbiased inventory of human and avian RNA binding proteins (RBPs) co-isolating with 5'UTRs of HIV-1, spleen necrosis virus and Rous sarcoma virus. Applying stringent biochemical and statistical criteria, we identified 185 RBP; 122 were previously implicated in retrovirus biology and 63 are new to the 5'UTR proteome. RNA electrophoretic mobility assays investigated paralogs present in the common ancestor of vertebrates and one hnRNP was identified as a central node to the biological process-anchored networks of HIV-1, SNV, and RSV 5' UTR-proteomes. This comprehensive view of the host constituents of retroviral RNPs is broadly applicable to investigation of viral replication and antiviral response in both human and avian cell lineages.

LanguageEnglish (US)
Pages307-320
Number of pages14
JournalVirology
Volume486
DOIs
StatePublished - Dec 1 2015

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RNA-Binding Proteins
5' Untranslated Regions
HIV-1
Chickens
Retroviridae
Proteome
Trager duck spleen necrosis virus
Avian Proteins
RNA
Heterogeneous-Nuclear Ribonucleoproteins
Biological Phenomena
Rous sarcoma virus
Cell Lineage
Proteomics
Antiviral Agents
Vertebrates
Viruses
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Virology

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Stake, M., Singh, D., Singh, G., Marcela Hernandez, J., Kaddis Maldonado, R., Parent, L. J., & Boris-Lawrie, K. (2015). HIV-1 and two avian retroviral 5' untranslated regions bind orthologous human and chicken RNA binding proteins. DOI: 10.1016/j.virol.2015.06.001
Stake, Matthew ; Singh, Deepali ; Singh, Gatikrushna ; Marcela Hernandez, J. ; Kaddis Maldonado, Rebecca ; Parent, Leslie J. ; Boris-Lawrie, Kathleen. / HIV-1 and two avian retroviral 5' untranslated regions bind orthologous human and chicken RNA binding proteins. In: Virology. 2015 ; Vol. 486. pp. 307-320
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Stake, M, Singh, D, Singh, G, Marcela Hernandez, J, Kaddis Maldonado, R, Parent, LJ & Boris-Lawrie, K 2015, 'HIV-1 and two avian retroviral 5' untranslated regions bind orthologous human and chicken RNA binding proteins' Virology, vol. 486, pp. 307-320. DOI: 10.1016/j.virol.2015.06.001

HIV-1 and two avian retroviral 5' untranslated regions bind orthologous human and chicken RNA binding proteins. / Stake, Matthew; Singh, Deepali; Singh, Gatikrushna; Marcela Hernandez, J.; Kaddis Maldonado, Rebecca; Parent, Leslie J.; Boris-Lawrie, Kathleen.

In: Virology, Vol. 486, 01.12.2015, p. 307-320.

Research output: Contribution to journalArticle

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Stake M, Singh D, Singh G, Marcela Hernandez J, Kaddis Maldonado R, Parent LJ et al. HIV-1 and two avian retroviral 5' untranslated regions bind orthologous human and chicken RNA binding proteins. Virology. 2015 Dec 1;486:307-320. Available from, DOI: 10.1016/j.virol.2015.06.001