HLA-JY328: Mapping studies and expression of a polymorphic HLA class I gene

Barry W. Duceman, Dolly Ness, Roberto Rende, Michael J. Chorney, Rakesh Srivastava, Daniel S. Greenspan, Julian Pan, Sherman M. Weissman, F. Carl Grumet

Research output: Contribution to journalArticle

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Abstract

The JY328 clone was identified in a human genomic library using cDNA corresponding to mRNA for HLA-B7 as a probe. The L/328 cell line was established by cotransformation of mouse Ltk- cells with the herpes thymidine kinase gene and clone JY328. On Northern blots, RNA from,L/328 strongly hybridized to an HLA class I probe, and an antigen was recognized by an anti-HLA class I framework antibody on the cell surface. A DNA probe corresponding to a segment of intron 7 was developed by comparing the nucleotide sequence of clone JY328 with that of other HLA class I-type genes. Using the radiolabeled probe to screen Southern blots of DNA from families with siblings exhibiting intra-HLA recombinations, a restriction fragment length polymorphism was revealed -a 1.4 kb BstE II band not present in all individuals. A corresponding fragment was apparent in the base sequence of clone JY328. The occurrence of this band on Southern blots established that JY328 maps distinct from and centromeric to the HLA-C locus and near to the HLA-B locus. Antibody absorption studies and cytotoxicity tests indicated that the JY328 gene product was not an HLA-B antigen but that it did specifically absorb CW7-specific antibody. In sum, these results suggest a novel, polymorphic HLA class I gene which expresses a product serologically similar to HLA-Cw7 but which does not map within the corresponding locus.

Original languageEnglish (US)
Pages (from-to)90-99
Number of pages10
JournalImmunogenetics
Volume23
Issue number2
DOIs
StatePublished - Feb 1 1986

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MHC Class I Genes
Clone Cells
HLA-B Antigens
Southern Blotting
Antibodies
HLA-B7 Antigen
HLA-C Antigens
Histocompatibility Antigens Class I
Genomic Library
Thymidine Kinase
DNA Probes
Restriction Fragment Length Polymorphisms
Northern Blotting
Introns
Genetic Recombination
Genes
Complementary DNA
RNA
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Duceman, B. W., Ness, D., Rende, R., Chorney, M. J., Srivastava, R., Greenspan, D. S., ... Grumet, F. C. (1986). HLA-JY328: Mapping studies and expression of a polymorphic HLA class I gene. Immunogenetics, 23(2), 90-99. https://doi.org/10.1007/BF00377967
Duceman, Barry W. ; Ness, Dolly ; Rende, Roberto ; Chorney, Michael J. ; Srivastava, Rakesh ; Greenspan, Daniel S. ; Pan, Julian ; Weissman, Sherman M. ; Grumet, F. Carl. / HLA-JY328 : Mapping studies and expression of a polymorphic HLA class I gene. In: Immunogenetics. 1986 ; Vol. 23, No. 2. pp. 90-99.
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abstract = "The JY328 clone was identified in a human genomic library using cDNA corresponding to mRNA for HLA-B7 as a probe. The L/328 cell line was established by cotransformation of mouse Ltk- cells with the herpes thymidine kinase gene and clone JY328. On Northern blots, RNA from,L/328 strongly hybridized to an HLA class I probe, and an antigen was recognized by an anti-HLA class I framework antibody on the cell surface. A DNA probe corresponding to a segment of intron 7 was developed by comparing the nucleotide sequence of clone JY328 with that of other HLA class I-type genes. Using the radiolabeled probe to screen Southern blots of DNA from families with siblings exhibiting intra-HLA recombinations, a restriction fragment length polymorphism was revealed -a 1.4 kb BstE II band not present in all individuals. A corresponding fragment was apparent in the base sequence of clone JY328. The occurrence of this band on Southern blots established that JY328 maps distinct from and centromeric to the HLA-C locus and near to the HLA-B locus. Antibody absorption studies and cytotoxicity tests indicated that the JY328 gene product was not an HLA-B antigen but that it did specifically absorb CW7-specific antibody. In sum, these results suggest a novel, polymorphic HLA class I gene which expresses a product serologically similar to HLA-Cw7 but which does not map within the corresponding locus.",
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Duceman, BW, Ness, D, Rende, R, Chorney, MJ, Srivastava, R, Greenspan, DS, Pan, J, Weissman, SM & Grumet, FC 1986, 'HLA-JY328: Mapping studies and expression of a polymorphic HLA class I gene', Immunogenetics, vol. 23, no. 2, pp. 90-99. https://doi.org/10.1007/BF00377967

HLA-JY328 : Mapping studies and expression of a polymorphic HLA class I gene. / Duceman, Barry W.; Ness, Dolly; Rende, Roberto; Chorney, Michael J.; Srivastava, Rakesh; Greenspan, Daniel S.; Pan, Julian; Weissman, Sherman M.; Grumet, F. Carl.

In: Immunogenetics, Vol. 23, No. 2, 01.02.1986, p. 90-99.

Research output: Contribution to journalArticle

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T2 - Mapping studies and expression of a polymorphic HLA class I gene

AU - Duceman, Barry W.

AU - Ness, Dolly

AU - Rende, Roberto

AU - Chorney, Michael J.

AU - Srivastava, Rakesh

AU - Greenspan, Daniel S.

AU - Pan, Julian

AU - Weissman, Sherman M.

AU - Grumet, F. Carl

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N2 - The JY328 clone was identified in a human genomic library using cDNA corresponding to mRNA for HLA-B7 as a probe. The L/328 cell line was established by cotransformation of mouse Ltk- cells with the herpes thymidine kinase gene and clone JY328. On Northern blots, RNA from,L/328 strongly hybridized to an HLA class I probe, and an antigen was recognized by an anti-HLA class I framework antibody on the cell surface. A DNA probe corresponding to a segment of intron 7 was developed by comparing the nucleotide sequence of clone JY328 with that of other HLA class I-type genes. Using the radiolabeled probe to screen Southern blots of DNA from families with siblings exhibiting intra-HLA recombinations, a restriction fragment length polymorphism was revealed -a 1.4 kb BstE II band not present in all individuals. A corresponding fragment was apparent in the base sequence of clone JY328. The occurrence of this band on Southern blots established that JY328 maps distinct from and centromeric to the HLA-C locus and near to the HLA-B locus. Antibody absorption studies and cytotoxicity tests indicated that the JY328 gene product was not an HLA-B antigen but that it did specifically absorb CW7-specific antibody. In sum, these results suggest a novel, polymorphic HLA class I gene which expresses a product serologically similar to HLA-Cw7 but which does not map within the corresponding locus.

AB - The JY328 clone was identified in a human genomic library using cDNA corresponding to mRNA for HLA-B7 as a probe. The L/328 cell line was established by cotransformation of mouse Ltk- cells with the herpes thymidine kinase gene and clone JY328. On Northern blots, RNA from,L/328 strongly hybridized to an HLA class I probe, and an antigen was recognized by an anti-HLA class I framework antibody on the cell surface. A DNA probe corresponding to a segment of intron 7 was developed by comparing the nucleotide sequence of clone JY328 with that of other HLA class I-type genes. Using the radiolabeled probe to screen Southern blots of DNA from families with siblings exhibiting intra-HLA recombinations, a restriction fragment length polymorphism was revealed -a 1.4 kb BstE II band not present in all individuals. A corresponding fragment was apparent in the base sequence of clone JY328. The occurrence of this band on Southern blots established that JY328 maps distinct from and centromeric to the HLA-C locus and near to the HLA-B locus. Antibody absorption studies and cytotoxicity tests indicated that the JY328 gene product was not an HLA-B antigen but that it did specifically absorb CW7-specific antibody. In sum, these results suggest a novel, polymorphic HLA class I gene which expresses a product serologically similar to HLA-Cw7 but which does not map within the corresponding locus.

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Duceman BW, Ness D, Rende R, Chorney MJ, Srivastava R, Greenspan DS et al. HLA-JY328: Mapping studies and expression of a polymorphic HLA class I gene. Immunogenetics. 1986 Feb 1;23(2):90-99. https://doi.org/10.1007/BF00377967