HLA-JY328: Mapping studies and expression of a polymorphic HLA class I gene

Barry W. Duceman, Dolly Ness, Roberto Rende, Michael J. Chorney, Rakesh Srivastava, Daniel S. Greenspan, Julian Pan, Sherman M. Weissman, F. Carl Grumet

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20 Scopus citations

Abstract

The JY328 clone was identified in a human genomic library using cDNA corresponding to mRNA for HLA-B7 as a probe. The L/328 cell line was established by cotransformation of mouse Ltk- cells with the herpes thymidine kinase gene and clone JY328. On Northern blots, RNA from,L/328 strongly hybridized to an HLA class I probe, and an antigen was recognized by an anti-HLA class I framework antibody on the cell surface. A DNA probe corresponding to a segment of intron 7 was developed by comparing the nucleotide sequence of clone JY328 with that of other HLA class I-type genes. Using the radiolabeled probe to screen Southern blots of DNA from families with siblings exhibiting intra-HLA recombinations, a restriction fragment length polymorphism was revealed -a 1.4 kb BstE II band not present in all individuals. A corresponding fragment was apparent in the base sequence of clone JY328. The occurrence of this band on Southern blots established that JY328 maps distinct from and centromeric to the HLA-C locus and near to the HLA-B locus. Antibody absorption studies and cytotoxicity tests indicated that the JY328 gene product was not an HLA-B antigen but that it did specifically absorb CW7-specific antibody. In sum, these results suggest a novel, polymorphic HLA class I gene which expresses a product serologically similar to HLA-Cw7 but which does not map within the corresponding locus.

Original languageEnglish (US)
Pages (from-to)90-99
Number of pages10
JournalImmunogenetics
Volume23
Issue number2
DOIs
StatePublished - Feb 1 1986

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

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