Hormonal and immunological regulation of 2′,5′-oligoadenylate synthetase activity in human peripheral blood mononuclear cells

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Abstract

A newly developed method for assaying 2′,5′-oligoadenylate (2,5A) synthetase activity by polyacrylamide gel electrophoresis was applied to peripheral blood mononuclear cells (PBMC) from normal subjects, HIV-positive subjects, and renal cell carcinoma (RCC) patients. Sex differences were observed in 2,5A synthetase activity of PBMC from normal young adults, males having eightfold higher activities of this enzyme than females. Moreover, compared to values for postmenopausal (PM) females receiving estrogen replacement, untreated PM females had higher activities. Collectively, these results suggest that estrogen downregulates 2,5A synthetase activity. Activities of 2,5A synthetase were investigated in two disease states associated with altered immune function. In one patient with AIDS-related Kaposi's sarcoma, interferon-α (IFN-α) therapy increased 2,5A synthetase activity twofold. In addition, combined therapy with interleukin-2 (IL-2) and IFN-α increased 2,5A synthetase activities in eight of nine patients with RCC. Therefore, in patients receiving immunotherapy with IL-2 and IFN-α, our new assay could contribute to evaluation of immune stimulation. In general, studies in vitro confirmed these observations; however, exposure of PBMC from RCC patients revealed that in vitro IL-2 failed to induce this enzyme activity as it did in PBMC from normal volunteers.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalClinical Immunology and Immunopathology
Volume65
Issue number2
DOIs
StatePublished - Jan 1 1992

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Ligases
Human Activities
Blood Cells
Renal Cell Carcinoma
Interferons
Interleukin-2
Estrogen Replacement Therapy
Enzymes
Sex Characteristics
Immunotherapy
2',5'-oligoadenylate
Polyacrylamide Gel Electrophoresis
Young Adult
Healthy Volunteers
Estrogens
Down-Regulation
HIV
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

Cite this

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title = "Hormonal and immunological regulation of 2′,5′-oligoadenylate synthetase activity in human peripheral blood mononuclear cells",
abstract = "A newly developed method for assaying 2′,5′-oligoadenylate (2,5A) synthetase activity by polyacrylamide gel electrophoresis was applied to peripheral blood mononuclear cells (PBMC) from normal subjects, HIV-positive subjects, and renal cell carcinoma (RCC) patients. Sex differences were observed in 2,5A synthetase activity of PBMC from normal young adults, males having eightfold higher activities of this enzyme than females. Moreover, compared to values for postmenopausal (PM) females receiving estrogen replacement, untreated PM females had higher activities. Collectively, these results suggest that estrogen downregulates 2,5A synthetase activity. Activities of 2,5A synthetase were investigated in two disease states associated with altered immune function. In one patient with AIDS-related Kaposi's sarcoma, interferon-α (IFN-α) therapy increased 2,5A synthetase activity twofold. In addition, combined therapy with interleukin-2 (IL-2) and IFN-α increased 2,5A synthetase activities in eight of nine patients with RCC. Therefore, in patients receiving immunotherapy with IL-2 and IFN-α, our new assay could contribute to evaluation of immune stimulation. In general, studies in vitro confirmed these observations; however, exposure of PBMC from RCC patients revealed that in vitro IL-2 failed to induce this enzyme activity as it did in PBMC from normal volunteers.",
author = "Miele, {Mary Beth} and Vesell, {E. S.} and Ehmann, {W. Christopher} and Allan Lipton and Harold Harvey and Kan, {N. C.}",
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T1 - Hormonal and immunological regulation of 2′,5′-oligoadenylate synthetase activity in human peripheral blood mononuclear cells

AU - Miele, Mary Beth

AU - Vesell, E. S.

AU - Ehmann, W. Christopher

AU - Lipton, Allan

AU - Harvey, Harold

AU - Kan, N. C.

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AB - A newly developed method for assaying 2′,5′-oligoadenylate (2,5A) synthetase activity by polyacrylamide gel electrophoresis was applied to peripheral blood mononuclear cells (PBMC) from normal subjects, HIV-positive subjects, and renal cell carcinoma (RCC) patients. Sex differences were observed in 2,5A synthetase activity of PBMC from normal young adults, males having eightfold higher activities of this enzyme than females. Moreover, compared to values for postmenopausal (PM) females receiving estrogen replacement, untreated PM females had higher activities. Collectively, these results suggest that estrogen downregulates 2,5A synthetase activity. Activities of 2,5A synthetase were investigated in two disease states associated with altered immune function. In one patient with AIDS-related Kaposi's sarcoma, interferon-α (IFN-α) therapy increased 2,5A synthetase activity twofold. In addition, combined therapy with interleukin-2 (IL-2) and IFN-α increased 2,5A synthetase activities in eight of nine patients with RCC. Therefore, in patients receiving immunotherapy with IL-2 and IFN-α, our new assay could contribute to evaluation of immune stimulation. In general, studies in vitro confirmed these observations; however, exposure of PBMC from RCC patients revealed that in vitro IL-2 failed to induce this enzyme activity as it did in PBMC from normal volunteers.

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