Hormonal control of vitellogenesis in the gypsy moth, Lymantria dispar (L.): Suppression of haemolymph vitellogenin by the juvenile hormone analogue, methoprene

Robin E. Davis, Thomas J. Kelly, Edward P. Masler, Howard William Fescemyer, Belgaum S. Thyagaraja, Alexej B. Borkovec

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In most insects, female-specific egg-protein precursors (vitellogenins) are synthesized during pupal or adult stages in response to 20-hydroxyecdysone and/or juvenile hormone. In the gypsy moth, two female-specific haemolymph polypeptides (apo-vitellogenins) having electrophoretic and immunologic identity with Lymantria dispar egg-vitellin apo-proteins begin to accumulate in the haemolymph after day 3 of the fifth larval instar. Head ligation, starvation or treatment with doses of a juvenile hormone analogue, methoprene, >10 μg prior to day 3 block the accumulation of apo-vitellogenin in the haemolymph. Unlike head-ligated or starved larvae which do not gain weight and do no accumulate normal levels of non-vitellogenin haemolymph proteins following treatment, methoprene-treated larvae grow normally and accumulate non-vitellogenin haemolymph proteins. 20-Hydroxyecdysone (2.5 μg) has no effect on the haemolymph accumulation of apo-vitellogenins in normal larvae, and does not restore haemolymph apo-vitellogenins in methoprene-treated, head-ligated or starved larvae. The above results suggest that a low (or declining) juvenile hormone titre during a critical stage early in the last larval instar (prior to day 3) is necessary for the accumulation of vitellogenin in the haemolymph to proceed normally in the gypsy moth. Other factors such as nutrient intake may also be involved. This role of juvenile hormone as an inhibitor of vitellogenin accumulation/synthesis is a novel one for insect species.

Original languageEnglish (US)
Pages (from-to)231-238
Number of pages8
JournalJournal of Insect Physiology
Volume36
Issue number4
DOIs
StatePublished - Jan 1 1990

Fingerprint

Methoprene
Vitellogenesis
Vitellogenins
Juvenile Hormones
methoprene
juvenile hormone analogs
Hemolymph
Moths
Lymantria dispar
hormonal regulation
vitellogenin
vitellogenesis
hemolymph
Larva
juvenile hormones
Ecdysterone
ecdysterone
larvae
Head
Insects

All Science Journal Classification (ASJC) codes

  • Physiology
  • Insect Science

Cite this

Davis, Robin E. ; Kelly, Thomas J. ; Masler, Edward P. ; Fescemyer, Howard William ; Thyagaraja, Belgaum S. ; Borkovec, Alexej B. / Hormonal control of vitellogenesis in the gypsy moth, Lymantria dispar (L.) : Suppression of haemolymph vitellogenin by the juvenile hormone analogue, methoprene. In: Journal of Insect Physiology. 1990 ; Vol. 36, No. 4. pp. 231-238.
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abstract = "In most insects, female-specific egg-protein precursors (vitellogenins) are synthesized during pupal or adult stages in response to 20-hydroxyecdysone and/or juvenile hormone. In the gypsy moth, two female-specific haemolymph polypeptides (apo-vitellogenins) having electrophoretic and immunologic identity with Lymantria dispar egg-vitellin apo-proteins begin to accumulate in the haemolymph after day 3 of the fifth larval instar. Head ligation, starvation or treatment with doses of a juvenile hormone analogue, methoprene, >10 μg prior to day 3 block the accumulation of apo-vitellogenin in the haemolymph. Unlike head-ligated or starved larvae which do not gain weight and do no accumulate normal levels of non-vitellogenin haemolymph proteins following treatment, methoprene-treated larvae grow normally and accumulate non-vitellogenin haemolymph proteins. 20-Hydroxyecdysone (2.5 μg) has no effect on the haemolymph accumulation of apo-vitellogenins in normal larvae, and does not restore haemolymph apo-vitellogenins in methoprene-treated, head-ligated or starved larvae. The above results suggest that a low (or declining) juvenile hormone titre during a critical stage early in the last larval instar (prior to day 3) is necessary for the accumulation of vitellogenin in the haemolymph to proceed normally in the gypsy moth. Other factors such as nutrient intake may also be involved. This role of juvenile hormone as an inhibitor of vitellogenin accumulation/synthesis is a novel one for insect species.",
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Hormonal control of vitellogenesis in the gypsy moth, Lymantria dispar (L.) : Suppression of haemolymph vitellogenin by the juvenile hormone analogue, methoprene. / Davis, Robin E.; Kelly, Thomas J.; Masler, Edward P.; Fescemyer, Howard William; Thyagaraja, Belgaum S.; Borkovec, Alexej B.

In: Journal of Insect Physiology, Vol. 36, No. 4, 01.01.1990, p. 231-238.

Research output: Contribution to journalArticle

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AU - Davis, Robin E.

AU - Kelly, Thomas J.

AU - Masler, Edward P.

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N2 - In most insects, female-specific egg-protein precursors (vitellogenins) are synthesized during pupal or adult stages in response to 20-hydroxyecdysone and/or juvenile hormone. In the gypsy moth, two female-specific haemolymph polypeptides (apo-vitellogenins) having electrophoretic and immunologic identity with Lymantria dispar egg-vitellin apo-proteins begin to accumulate in the haemolymph after day 3 of the fifth larval instar. Head ligation, starvation or treatment with doses of a juvenile hormone analogue, methoprene, >10 μg prior to day 3 block the accumulation of apo-vitellogenin in the haemolymph. Unlike head-ligated or starved larvae which do not gain weight and do no accumulate normal levels of non-vitellogenin haemolymph proteins following treatment, methoprene-treated larvae grow normally and accumulate non-vitellogenin haemolymph proteins. 20-Hydroxyecdysone (2.5 μg) has no effect on the haemolymph accumulation of apo-vitellogenins in normal larvae, and does not restore haemolymph apo-vitellogenins in methoprene-treated, head-ligated or starved larvae. The above results suggest that a low (or declining) juvenile hormone titre during a critical stage early in the last larval instar (prior to day 3) is necessary for the accumulation of vitellogenin in the haemolymph to proceed normally in the gypsy moth. Other factors such as nutrient intake may also be involved. This role of juvenile hormone as an inhibitor of vitellogenin accumulation/synthesis is a novel one for insect species.

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