Hormone dependency of human breast neoplasma cultured in vitro in the stem cell assay

Andrea Manni, C. Wright, M. Pontari, P. Feil, R. Joehl

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The stem cell soft agar culture system provides a biological tool with which to study tumor clones derived from heterogeneous tumor cell populations. For testing the feasibility of this approach in indentifying hormone-responsive clones of mammary tumors, a study was done to determine whether estrogen deprivation or supplementation would alter colony formation in sequential breast neoplasms received in the Cell Kinetics Laboratory, The Pennsylvania State University. Single-cell suspensions obtained from 22 freshly excised breast neoplasms (20 malignant, 2 benign) were plated in soft agar with and without tamoxifen (Tam) (10-7 M) with the use of serum-supplemented media and with and without 17β-estradiol (E2) (10-8 M) under serum-free medium conditions. Twenty tumors successfully grew, producing 42 ± 8 (mean ± SEM) colonies in control dishes in the presence of serum and 27 ± 5 in its absence (P < .001). Tam inhibited colony formation in 78% of malignant tumors, whereas a stimulatory effect was observed with E2 in 94%. An increase in colony formation was induced by Tam in 4 tumors (2 benign and 2 malignant). The effects of E2 and Tam on tumor growth were not influenced by the estrogen and prosgesterone receptor status of the tumor. These preliminary results suggest that all tumors, irrespective of their receptor status, may contain clones of hormone-responsive cells.

Original languageEnglish (US)
Pages (from-to)767-770
Number of pages4
JournalJournal of the National Cancer Institute
Volume74
Issue number4
StatePublished - Jul 11 1985

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Colony-Forming Units Assay
Breast
Hormones
Tamoxifen
Neoplasms
Clone Cells
Breast Neoplasms
Agar
In Vitro Techniques
Serum-Free Culture Media
Serum
Estrogen Receptors
Estradiol
Suspensions
Estrogens
Stem Cells

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Manni, Andrea ; Wright, C. ; Pontari, M. ; Feil, P. ; Joehl, R. / Hormone dependency of human breast neoplasma cultured in vitro in the stem cell assay. In: Journal of the National Cancer Institute. 1985 ; Vol. 74, No. 4. pp. 767-770.
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Hormone dependency of human breast neoplasma cultured in vitro in the stem cell assay. / Manni, Andrea; Wright, C.; Pontari, M.; Feil, P.; Joehl, R.

In: Journal of the National Cancer Institute, Vol. 74, No. 4, 11.07.1985, p. 767-770.

Research output: Contribution to journalArticle

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