Host genetic determinants of microbiota-dependent nutrition revealed by genome-wide analysis of Drosophila melanogaster

Adam J. Dobson, John M. Chaston, Peter D. Newell, Leanne Donahue, Sara L. Hermann, David R. Sannino, Stephanie Westmiller, Adam C.N. Wong, Andrew G. Clark, Brian P. Lazzaro, Angela E. Douglas

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Animals bear communities of gut microorganisms with substantial effects on animal nutrition, but the host genetic basis of these effects is unknown. Here we use Drosophila to demonstrate substantial among-genotype variation in the effects of eliminating the gut microbiota on five host nutritional indices (weight, protein, lipid, glucose and glycogen contents); this includes variation in both the magnitude and direction of microbiota-dependent effects. Genome-wide association studies to identify the genetic basis of the microbiota-dependent variation reveal polymorphisms in largely non-overlapping sets of genes associated with variation in the nutritional traits, including strong representation of conserved genes functioning in signalling. Key genes identified by the GWA study are validated by loss-of-function mutations that altered microbiota-dependent nutritional effects. We conclude that the microbiota interacts with the animal at multiple points in the signalling and regulatory networks that determine animal nutrition. These interactions with the microbiota are probably conserved across animals, including humans.

Original languageEnglish (US)
Article number6312
JournalNature communications
Volume6
DOIs
StatePublished - Feb 18 2015

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nutrition
Drosophila
genome
Microbiota
Nutrition
Drosophila melanogaster
determinants
animals
Animals
Genes
Genome
genes
Genome-Wide Association Study
glycogens
Nutrition Assessment
polymorphism
microorganisms
mutations
bears
Polymorphism

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Dobson, Adam J. ; Chaston, John M. ; Newell, Peter D. ; Donahue, Leanne ; Hermann, Sara L. ; Sannino, David R. ; Westmiller, Stephanie ; Wong, Adam C.N. ; Clark, Andrew G. ; Lazzaro, Brian P. ; Douglas, Angela E. / Host genetic determinants of microbiota-dependent nutrition revealed by genome-wide analysis of Drosophila melanogaster. In: Nature communications. 2015 ; Vol. 6.
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abstract = "Animals bear communities of gut microorganisms with substantial effects on animal nutrition, but the host genetic basis of these effects is unknown. Here we use Drosophila to demonstrate substantial among-genotype variation in the effects of eliminating the gut microbiota on five host nutritional indices (weight, protein, lipid, glucose and glycogen contents); this includes variation in both the magnitude and direction of microbiota-dependent effects. Genome-wide association studies to identify the genetic basis of the microbiota-dependent variation reveal polymorphisms in largely non-overlapping sets of genes associated with variation in the nutritional traits, including strong representation of conserved genes functioning in signalling. Key genes identified by the GWA study are validated by loss-of-function mutations that altered microbiota-dependent nutritional effects. We conclude that the microbiota interacts with the animal at multiple points in the signalling and regulatory networks that determine animal nutrition. These interactions with the microbiota are probably conserved across animals, including humans.",
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Dobson, AJ, Chaston, JM, Newell, PD, Donahue, L, Hermann, SL, Sannino, DR, Westmiller, S, Wong, ACN, Clark, AG, Lazzaro, BP & Douglas, AE 2015, 'Host genetic determinants of microbiota-dependent nutrition revealed by genome-wide analysis of Drosophila melanogaster', Nature communications, vol. 6, 6312. https://doi.org/10.1038/ncomms7312

Host genetic determinants of microbiota-dependent nutrition revealed by genome-wide analysis of Drosophila melanogaster. / Dobson, Adam J.; Chaston, John M.; Newell, Peter D.; Donahue, Leanne; Hermann, Sara L.; Sannino, David R.; Westmiller, Stephanie; Wong, Adam C.N.; Clark, Andrew G.; Lazzaro, Brian P.; Douglas, Angela E.

In: Nature communications, Vol. 6, 6312, 18.02.2015.

Research output: Contribution to journalArticle

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