Host miRNAs are involved in hormonal regulation of HaSNPV-triggered climbing behaviour in Helicoverpa armigera

Songdou Zhang, Shiheng An, Kelli Hoover, Zhen Li, Xiangrui Li, Xiaoming Liu, Zhongjian Shen, Haibo Fang, Vera I.D. Ros, Qingwen Zhang, Xiaoxia Liu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Baculoviruses manipulate host climbing behaviour to ensure that the hosts die at elevated positions on host plants to facilitate virus proliferation and transmission, which is a process referred to as tree-top disease. However, the detailed molecular mechanism underlying tree-top disease has not been elucidated. Using transcriptome analysis, we showed that two hormone signals, juvenile hormone (JH) and 20-hydroxyecdysone (20E), are key components involved in HaSNPV-induced tree-top disease in Helicoverpa armigera larvae. RNAi-mediated knockdown and exogenous hormone treatment assays demonstrated that 20E inhibits virus-induced tree-top disease, while JH mediates tree-top disease behaviour. Knockdown of BrZ2, a downstream signal of JH and 20E, promoted HaSNPV-induced tree-top disease. We also found that two miRNAs target BrZ2 and are involved in the cross-talk regulation between 20E and JH manipulating HaSNPV replication, time to death and HaSNPV-induced tree-top disease.

Original languageEnglish (US)
Pages (from-to)459-475
Number of pages17
JournalMolecular ecology
Volume27
Issue number2
DOIs
StatePublished - Jan 1 2018

Fingerprint

hormonal regulation
Helicoverpa armigera
MicroRNAs
tree crown
hormone
Juvenile Hormones
juvenile hormones
virus
hormones
Hormones
Ecdysterone
Viruses
viruses
ecdysterone
Baculoviridae
Gene Expression Profiling
RNA Interference
regulation
transcriptomics
host plant

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Zhang, Songdou ; An, Shiheng ; Hoover, Kelli ; Li, Zhen ; Li, Xiangrui ; Liu, Xiaoming ; Shen, Zhongjian ; Fang, Haibo ; Ros, Vera I.D. ; Zhang, Qingwen ; Liu, Xiaoxia. / Host miRNAs are involved in hormonal regulation of HaSNPV-triggered climbing behaviour in Helicoverpa armigera. In: Molecular ecology. 2018 ; Vol. 27, No. 2. pp. 459-475.
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title = "Host miRNAs are involved in hormonal regulation of HaSNPV-triggered climbing behaviour in Helicoverpa armigera",
abstract = "Baculoviruses manipulate host climbing behaviour to ensure that the hosts die at elevated positions on host plants to facilitate virus proliferation and transmission, which is a process referred to as tree-top disease. However, the detailed molecular mechanism underlying tree-top disease has not been elucidated. Using transcriptome analysis, we showed that two hormone signals, juvenile hormone (JH) and 20-hydroxyecdysone (20E), are key components involved in HaSNPV-induced tree-top disease in Helicoverpa armigera larvae. RNAi-mediated knockdown and exogenous hormone treatment assays demonstrated that 20E inhibits virus-induced tree-top disease, while JH mediates tree-top disease behaviour. Knockdown of BrZ2, a downstream signal of JH and 20E, promoted HaSNPV-induced tree-top disease. We also found that two miRNAs target BrZ2 and are involved in the cross-talk regulation between 20E and JH manipulating HaSNPV replication, time to death and HaSNPV-induced tree-top disease.",
author = "Songdou Zhang and Shiheng An and Kelli Hoover and Zhen Li and Xiangrui Li and Xiaoming Liu and Zhongjian Shen and Haibo Fang and Ros, {Vera I.D.} and Qingwen Zhang and Xiaoxia Liu",
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Zhang, S, An, S, Hoover, K, Li, Z, Li, X, Liu, X, Shen, Z, Fang, H, Ros, VID, Zhang, Q & Liu, X 2018, 'Host miRNAs are involved in hormonal regulation of HaSNPV-triggered climbing behaviour in Helicoverpa armigera', Molecular ecology, vol. 27, no. 2, pp. 459-475. https://doi.org/10.1111/mec.14457

Host miRNAs are involved in hormonal regulation of HaSNPV-triggered climbing behaviour in Helicoverpa armigera. / Zhang, Songdou; An, Shiheng; Hoover, Kelli; Li, Zhen; Li, Xiangrui; Liu, Xiaoming; Shen, Zhongjian; Fang, Haibo; Ros, Vera I.D.; Zhang, Qingwen; Liu, Xiaoxia.

In: Molecular ecology, Vol. 27, No. 2, 01.01.2018, p. 459-475.

Research output: Contribution to journalArticle

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AU - Zhang, Songdou

AU - An, Shiheng

AU - Hoover, Kelli

AU - Li, Zhen

AU - Li, Xiangrui

AU - Liu, Xiaoming

AU - Shen, Zhongjian

AU - Fang, Haibo

AU - Ros, Vera I.D.

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AU - Liu, Xiaoxia

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