Host-plant induced changes in microbial community structure and midgut gene expression in an invasive polyphage (Anoplophora glabripennis

Erin D. Scully, Scott M. Geib, Charles J. Mason, John Edward Carlson, Ming Tien, Han Yi Chen, Scott Harding, Chung Jui Tsai, Kelli Hoover

Research output: Contribution to journalArticle

Abstract

Polyphagous insect herbivores possess diverse mechanisms to overcome challenges of feeding in multiple plant species including, but not limited to, transcriptional plasticity and associations with obligate or facultative symbionts. The Asian longhorned beetle (Anoplophora glabripennis) is a polyphagous wood-feeder capable of developing on over 100 tree species and, like other polyphages, its genome contains amplifications of digestive and detoxification genes. This insect also possesses a diverse gut microbial community, which has the metabolic potential to augment digestive physiology. While the genomic repertoires of A. glabripennis and its microbial community have been studied previously, comparatively less is known about how the gut transcriptome and community change in response to feeding in different hosts. In this study, we show that feeding in two suitable hosts (Acer spp. and Populus nigra) altered the expression levels of multicopy genes linked to digestion and detoxification. However, feeding in a host with documented resistance (Populus tomentosa) induced changes in the transcriptome and community beyond what was observed in insects reared in P. nigra, including the downregulation of numerous β-glucosidases, odorant binding proteins, and juvenile hormone binding proteins, the upregulation of several cuticular genes, and the loss of one major bacterial family from the gut community.

Original languageEnglish (US)
Article number9620
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Anoplophora glabripennis
midgut
microbial communities
Populus nigra
community structure
host plants
transcriptome
gene expression
insects
digestive system
Populus tomentosa
digestive physiology
glucosidases
genes
Acer
juvenile hormones
intestinal microorganisms
symbionts
binding proteins
herbivores

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "Host-plant induced changes in microbial community structure and midgut gene expression in an invasive polyphage (Anoplophora glabripennis",
abstract = "Polyphagous insect herbivores possess diverse mechanisms to overcome challenges of feeding in multiple plant species including, but not limited to, transcriptional plasticity and associations with obligate or facultative symbionts. The Asian longhorned beetle (Anoplophora glabripennis) is a polyphagous wood-feeder capable of developing on over 100 tree species and, like other polyphages, its genome contains amplifications of digestive and detoxification genes. This insect also possesses a diverse gut microbial community, which has the metabolic potential to augment digestive physiology. While the genomic repertoires of A. glabripennis and its microbial community have been studied previously, comparatively less is known about how the gut transcriptome and community change in response to feeding in different hosts. In this study, we show that feeding in two suitable hosts (Acer spp. and Populus nigra) altered the expression levels of multicopy genes linked to digestion and detoxification. However, feeding in a host with documented resistance (Populus tomentosa) induced changes in the transcriptome and community beyond what was observed in insects reared in P. nigra, including the downregulation of numerous β-glucosidases, odorant binding proteins, and juvenile hormone binding proteins, the upregulation of several cuticular genes, and the loss of one major bacterial family from the gut community.",
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Host-plant induced changes in microbial community structure and midgut gene expression in an invasive polyphage (Anoplophora glabripennis. / Scully, Erin D.; Geib, Scott M.; Mason, Charles J.; Carlson, John Edward; Tien, Ming; Chen, Han Yi; Harding, Scott; Tsai, Chung Jui; Hoover, Kelli.

In: Scientific reports, Vol. 8, No. 1, 9620, 01.12.2018.

Research output: Contribution to journalArticle

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AU - Geib, Scott M.

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AU - Tien, Ming

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