Human acylation stimulating protein enhances triacylglycerol biosynthesis in plant microsomes

Randall J. Weselake, E. Chris Kazala, Katherine Cianflone, David D. Boehr, Cameron K. Middleton, Courtney D. Rennie, André Laroche, Ingrid Recnik

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Diacylglycerol acyltransferase has a universal role in catalyzing the acyl-CoA-dependent formation of triacylglycerol in microorganisms, animals and plants. Acylation stimulating protein, from human blood, is known to enhance diacylglycerol acyltransferase activity and triacylglycerol biosynthesis in human adipocytes. In the current study, acylation stimulating protein was also shown to enhance diacylglycerol acyltransferase activity in microsomes from cell suspension cultures of oilseed rape. Enzyme stimulation occurred over the pH range of 6-9 but the degree of stimulation decreased with increasing ionic strength at pH 7.4. Varying acyl-CoA concentration did not affect the degree of stimulation. Membranes from triacylglycerol producing cells in plants and humans may have similar binding sites for acylation stimulating protein which have been preserved during molecular evolution. The results suggest that human acylation stimulating protein may be useful in modifying lipid biosynthesis in plants. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)189-192
Number of pages4
JournalFEBS Letters
Volume481
Issue number2
DOIs
StatePublished - Sep 15 2000

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Diacylglycerol O-Acyltransferase
Biosynthesis
Microsomes
Triglycerides
Acyl Coenzyme A
Oilseeds
Molecular Evolution
Plant Cells
Ionic strength
Adipocytes
Microorganisms
Osmolar Concentration
Suspensions
Animals
Blood
Cell Culture Techniques
Binding Sites
Membranes
Lipids
des-Arg-(77)-complement C3a

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Weselake, R. J., Kazala, E. C., Cianflone, K., Boehr, D. D., Middleton, C. K., Rennie, C. D., ... Recnik, I. (2000). Human acylation stimulating protein enhances triacylglycerol biosynthesis in plant microsomes. FEBS Letters, 481(2), 189-192. https://doi.org/10.1016/S0014-5793(00)01996-7
Weselake, Randall J. ; Kazala, E. Chris ; Cianflone, Katherine ; Boehr, David D. ; Middleton, Cameron K. ; Rennie, Courtney D. ; Laroche, André ; Recnik, Ingrid. / Human acylation stimulating protein enhances triacylglycerol biosynthesis in plant microsomes. In: FEBS Letters. 2000 ; Vol. 481, No. 2. pp. 189-192.
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Weselake, RJ, Kazala, EC, Cianflone, K, Boehr, DD, Middleton, CK, Rennie, CD, Laroche, A & Recnik, I 2000, 'Human acylation stimulating protein enhances triacylglycerol biosynthesis in plant microsomes', FEBS Letters, vol. 481, no. 2, pp. 189-192. https://doi.org/10.1016/S0014-5793(00)01996-7

Human acylation stimulating protein enhances triacylglycerol biosynthesis in plant microsomes. / Weselake, Randall J.; Kazala, E. Chris; Cianflone, Katherine; Boehr, David D.; Middleton, Cameron K.; Rennie, Courtney D.; Laroche, André; Recnik, Ingrid.

In: FEBS Letters, Vol. 481, No. 2, 15.09.2000, p. 189-192.

Research output: Contribution to journalArticle

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