Human alveolar macrophages release a factor that inhibits phagocyte function.

Y. Sibille, W. W. Merrill, G. P. Naegel, S. B. Care, J. A. Cooper, Herbert Reynolds

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human alveolar macrophages release in vitro a factor that inhibits both random migration and chemotaxis of human polymorphonuclear neutrophils (PMN). This factor is not cytotoxic and is recovered in culture supernatants of alveolar cells from most nonsmoking normal subjects. The inhibitor can be detected 30 min after cell cultures are established and is still produced after 24 h in culture. Its release was inhibited by cycloheximide. When supernatants are separated by molecular sieving (I-60 Waters HPLC column), most of the inhibitory activity is recovered in the low-molecular-weight fractions of the chromatogram (less than 1,000 D). The inhibitor has a broad spectrum of activity against known chemoattractants in that it reduces significantly the chemotaxis of PMN induced by the formyl peptide FMLP, by the complement fragment C5a, and by leukotriene B4; it also decreases the chemotactic activity associated with a monocyte-derived interleukin 1 preparation and the chemotactic activity derived from alveolar macrophage culture supernatants. The inhibitory factor is partially heat labile, is sensitive to aminopeptidase M, and is nonpolar. Both phorbol myristate acetate (PMA) and FMLP-induced superoxide release by PMN are diminished significantly in the presence of this inhibitory factor (p less than 0.01 for PMA and p less than 0.05 for FMLP). The inhibitor also reduces monocyte chemotaxis but has no effect on monocyte random migration. Finally, studies with [3H]FMLP indicate that this inhibitor does not act at the site of receptor binding on PMN. Thus, human alveolar macrophages can release in vitro both neutrophil chemotactic factors and an apparent neutrophil-inhibiting factor that may modulate positively and negatively the movement and the respiratory burst of neutrophils in the alveolar space.

Original languageEnglish (US)
Pages (from-to)407-416
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume1
Issue number5
DOIs
StatePublished - Jan 1 1989

Fingerprint

Alveolar Macrophages
Phagocytes
Neutrophils
Tetradecanoylphorbol Acetate
Chemotaxis
CD13 Antigens
Monocytes
Leukotriene B4
Chemotactic Factors
Cycloheximide
Interleukin-8
Interleukin-1
Cell culture
Superoxides
Molecular weight
Complement C5a
Alveolar Epithelial Cells
Peptides
Respiratory Burst
Water

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Sibille, Y. ; Merrill, W. W. ; Naegel, G. P. ; Care, S. B. ; Cooper, J. A. ; Reynolds, Herbert. / Human alveolar macrophages release a factor that inhibits phagocyte function. In: American Journal of Respiratory Cell and Molecular Biology. 1989 ; Vol. 1, No. 5. pp. 407-416.
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Human alveolar macrophages release a factor that inhibits phagocyte function. / Sibille, Y.; Merrill, W. W.; Naegel, G. P.; Care, S. B.; Cooper, J. A.; Reynolds, Herbert.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 1, No. 5, 01.01.1989, p. 407-416.

Research output: Contribution to journalArticle

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