Human cytosolic tRNase ZL can downregulate gene expression through miRNA

Reyad Elbarbary, Hiroaki Takaku, Naoto Uchiumi, Hiroko Tamiya, Mayumi Abe, Hiroshi Nishida, Masayuki Nashimoto

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including ∼25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3′ untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.

Original languageEnglish (US)
Pages (from-to)3241-3246
Number of pages6
JournalFEBS Letters
Volume583
Issue number19
DOIs
StatePublished - Oct 6 2009

Fingerprint

Guide RNA
MicroRNAs
Gene expression
Down-Regulation
Hooks
RNA Cleavage
RNA
Gene Expression
Messenger RNA
3' Untranslated Regions
Luciferases
Nucleotides

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Elbarbary, R., Takaku, H., Uchiumi, N., Tamiya, H., Abe, M., Nishida, H., & Nashimoto, M. (2009). Human cytosolic tRNase ZL can downregulate gene expression through miRNA. FEBS Letters, 583(19), 3241-3246. https://doi.org/10.1016/j.febslet.2009.09.015
Elbarbary, Reyad ; Takaku, Hiroaki ; Uchiumi, Naoto ; Tamiya, Hiroko ; Abe, Mayumi ; Nishida, Hiroshi ; Nashimoto, Masayuki. / Human cytosolic tRNase ZL can downregulate gene expression through miRNA. In: FEBS Letters. 2009 ; Vol. 583, No. 19. pp. 3241-3246.
@article{4c0fad84f6d34dd98ebb9edf81a55b93,
title = "Human cytosolic tRNase ZL can downregulate gene expression through miRNA",
abstract = "A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including ∼25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3′ untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.",
author = "Reyad Elbarbary and Hiroaki Takaku and Naoto Uchiumi and Hiroko Tamiya and Mayumi Abe and Hiroshi Nishida and Masayuki Nashimoto",
year = "2009",
month = "10",
day = "6",
doi = "10.1016/j.febslet.2009.09.015",
language = "English (US)",
volume = "583",
pages = "3241--3246",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "19",

}

Elbarbary, R, Takaku, H, Uchiumi, N, Tamiya, H, Abe, M, Nishida, H & Nashimoto, M 2009, 'Human cytosolic tRNase ZL can downregulate gene expression through miRNA', FEBS Letters, vol. 583, no. 19, pp. 3241-3246. https://doi.org/10.1016/j.febslet.2009.09.015

Human cytosolic tRNase ZL can downregulate gene expression through miRNA. / Elbarbary, Reyad; Takaku, Hiroaki; Uchiumi, Naoto; Tamiya, Hiroko; Abe, Mayumi; Nishida, Hiroshi; Nashimoto, Masayuki.

In: FEBS Letters, Vol. 583, No. 19, 06.10.2009, p. 3241-3246.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human cytosolic tRNase ZL can downregulate gene expression through miRNA

AU - Elbarbary, Reyad

AU - Takaku, Hiroaki

AU - Uchiumi, Naoto

AU - Tamiya, Hiroko

AU - Abe, Mayumi

AU - Nishida, Hiroshi

AU - Nashimoto, Masayuki

PY - 2009/10/6

Y1 - 2009/10/6

N2 - A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including ∼25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3′ untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.

AB - A long form of tRNase Z (tRNase ZL) can cleave any target RNA at any desired site under the direction of artificial small guide RNA including ∼25-nucleotide hook-shaped RNA. Here we show that human miR-103 can form a hook structure to guide target RNA cleavage by human cytosolic tRNase ZL in vitro. In vivo analyses using luciferase mRNAs modified to contain miR-103 target sequences in their 3′ untranslated regions indicated that miR-103 downregulates gene expression through directing mRNA cleavage by tRNase ZL. The present data suggest the possibility that human cytosolic tRNase ZL modulates gene expression through a subset of microRNAs in the cells.

UR - http://www.scopus.com/inward/record.url?scp=70349456506&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349456506&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2009.09.015

DO - 10.1016/j.febslet.2009.09.015

M3 - Article

VL - 583

SP - 3241

EP - 3246

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 19

ER -