Human embryonic and mesenchymal stem cells express different nuclear proteomes

Amritha Jaishankar, Miguel Barthelery, Willard M. Freeman, Ugur Salli, Timothy M. Ritty, Kent Vrana

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Human embryonic stem cells (hESCs) are characterized by their immortality and pluripotency. Human mesenchymal stem cells (hMSC), on the other hand, have limited self-renewal and differentiation capabilities. The underlying molecular differences that account for this characteristic self-renewal and plasticity are, however, poorly understood. This study reports a nuclear proteomic analysis of human embryonic and bone marrow-derived mesenchymal stem cells. Our proteomic screen highlighted a 5-fold difference in the expression of Reptin52. We show, using two-dimensional difference gel electrophoresis (2-DIGE), western analysis, and quantitative reverse transcriptase polymerase chain reaction, that Reptin52 is more abundantly expressed in hESC than hMSC. Moreover, we observed differential expression of Pontin52 and -catenin - proteins known to interact with Reptin52. This difference in the expression of Reptin52 and Pontin52 (known regulators of -catenin) further supports a role for Wnt signaling in stem cell self-renewal and proliferation.

Original languageEnglish (US)
Pages (from-to)793-801
Number of pages9
JournalStem Cells and Development
Volume18
Issue number5
DOIs
StatePublished - Jun 1 2009

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

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