Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing

Jung Hyun Kim, Thomas Ebersole, Natalay Kouprina, Vladimir N. Noskov, Jun Ichirou Ohzeki, Hiroshi Masumoto, Brankica Mravinac, Beth A. Sullivan, Adam Pavlicek, Sinisa Dovat, Svetlana D. Pack, Yoo Wook Kwon, Patrick T. Flanagan, Dmitri Loukinov, Victor Lobanenkov, Vladimir Larionov

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

The role of repetitive DNA sequences in pericentromeric regions with respect to kinetochore/heterochromatin structure and function is poorly understood. Here, we use a mouse erythroleukemia cell (MEL) system for studying how repetitive DNA assumes or is assembled into different chromatin structures. We show that human gamma-satellite DNA arrays allow a transcriptionally permissive chromatin conformation in an adjacent transgene and efficiently protect it from epigenetic silencing. These arrays contain CTCF and Ikaros binding sites. In MEL cells, this gamma-satellite DNA activity depends on binding of Ikaros proteins involved in differentiation along the hematopoietic pathway. Given our discovery of gamma-satellite DNA in pericentromeric regions of most human chromosomes and a dynamic chromatin state of gamma-satellite arrays in their natural location, we suggest that gamma-satellite DNA represents a unique region of the functional centromere with a possible role in preventing heterochromatin spreading beyond the pericentromeric region.

Original languageEnglish (US)
Pages (from-to)533-544
Number of pages12
JournalGenome research
Volume19
Issue number4
DOIs
StatePublished - Apr 1 2009

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing'. Together they form a unique fingerprint.

  • Cite this

    Kim, J. H., Ebersole, T., Kouprina, N., Noskov, V. N., Ohzeki, J. I., Masumoto, H., Mravinac, B., Sullivan, B. A., Pavlicek, A., Dovat, S., Pack, S. D., Kwon, Y. W., Flanagan, P. T., Loukinov, D., Lobanenkov, V., & Larionov, V. (2009). Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing. Genome research, 19(4), 533-544. https://doi.org/10.1101/gr.086496.108